"
Template:Team:UC Berkeley/Notebook/BX notes
From 2008.igem.org
| Line 2: | Line 2: | ||
==[[User:Sixpi|Sixpi]] 22:04, 11 June 2008 (UTC)== | ==[[User:Sixpi|Sixpi]] 22:04, 11 June 2008 (UTC)== | ||
| - | Our oligos arrived today(yay!). We started making our basic parts. I made a mistake while making the master mix - I didn't know the dNTPs were at 10 mM instead of 2 mM, so I added 5 times as much dNTPs than needed. This means our PCR reaction has a good chance of failing, since dNTPs bind Mg, which the DNA polymerase needs in order to copy DNA. The extra dNTPs will probably outcompete the polymerase. | + | Our oligos arrived today(yay!). We started making our basic parts. I made a mistake while making the master mix - I didn't know the dNTPs were at 10 mM instead of 2 mM, so I added 5 times as much dNTPs than needed. This means our PCR reaction has a good chance of failing, since dNTPs bind Mg, which the DNA polymerase needs in order to copy DNA. The extra dNTPs will probably outcompete the polymerase. No DNA was seen after running the eGel, so we set up the PCR reaction again, this time adding the correct amount of dNTPs. |
Yesterday I poured more plates; 1 liter of Amp/Kan, 1 liter of Amp/Cam, and 1 liter of Cam/Kan. | Yesterday I poured more plates; 1 liter of Amp/Kan, 1 liter of Amp/Cam, and 1 liter of Cam/Kan. | ||
Revision as of 01:34, 12 June 2008
Contents |
Sixpi 22:04, 11 June 2008 (UTC)
Our oligos arrived today(yay!). We started making our basic parts. I made a mistake while making the master mix - I didn't know the dNTPs were at 10 mM instead of 2 mM, so I added 5 times as much dNTPs than needed. This means our PCR reaction has a good chance of failing, since dNTPs bind Mg, which the DNA polymerase needs in order to copy DNA. The extra dNTPs will probably outcompete the polymerase. No DNA was seen after running the eGel, so we set up the PCR reaction again, this time adding the correct amount of dNTPs.
Yesterday I poured more plates; 1 liter of Amp/Kan, 1 liter of Amp/Cam, and 1 liter of Cam/Kan.
Sixpi 23:31, 9 June 2008 (UTC)
Today I poured plates. There were 3 liters of LB/agar media, to which I added Spe. The 3 liters made 150 plates.
Sixpi 00:02, 5 June 2008 (UTC)
In our demos this morning we transformed bacteria, plated them, picked colonies, grew them up, and extracted DNA. The afternoon was taken up by designing oligos for the basic parts will start making next week(hopefully).
Sixpi 05:12, 4 June 2008 (UTC)
Today we continued our part making demo. Yesterday we set up the PCR reaction, and today we cleaned up the PCR, set up the digestion, and also ran an analytical gel of the PCR product.
