Template:Team:UC Berkeley/Notebook/CC construction

From 2008.igem.org

(Difference between revisions)
Line 27: Line 27:
CgATAgaattcATGagatcttacccatacgacgtcccagac
CgATAgaattcATGagatcttacccatacgacgtcccagac
-
cc002             Reverse biobricking of {<HA!}
+
cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
</pre>
</pre>
Line 43: Line 43:
cc005            Reverse Biobricking of {<myc>}
cc005            Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG     
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG     
 +
</pre>
 +
 +
==K112504==
 +
<pre>
 +
Construction of {<myc!} basic part K112503
 +
 +
Wobble PCR of cc004/cc006                  (___bp, EcoRI/BamHI)
 +
Sub into pBca1256                          (EcoRI/BamHI)
 +
Product is pBca1256-K112504
 +
--------------------------------------------
 +
cc004            Forward Biobricking of {<myc>}
 +
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
 +
cc006            Reverse Biobricking of {<myc!}
 +
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC   
</pre>
</pre>

Revision as of 15:38, 24 June 2008

Contents


K112501

Construction of {<HA>} basic part K112501

Wobble PCR of cc001/cc002                  (___bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112501
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002             Reverse biobricking of {<HA>} 
ccagtggatccccagcgtagtctgggacgtcgtatggg

K112502

Construction of {<HA!} basic part K112502

Wobble PCR of cc001/cc003                  (___bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112502
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112503

Construction of {<myc>} basic part K112503

Wobble PCR of cc004/cc005                  (___bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112503
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005             Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG    

K112504

Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                  (___bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112504
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006             Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC