Template:Team:UC Berkeley/Notebook/CC construction

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(Difference between revisions)
(K112503)
(K112504)
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Construction of {<myc!} basic part K112503
Construction of {<myc!} basic part K112503
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Wobble PCR of cc004/cc006                  (___bp, EcoRI/BamHI)
+
Wobble PCR of cc004/cc006                  ( 64 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112504
Product is pBca1256-K112504

Revision as of 16:06, 24 June 2008

Contents


K112501

Construction of {<HA>} basic part K112501

Wobble PCR of cc001/cc002                  ( 60 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112501
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002             Reverse biobricking of {<HA>} 
ccagtggatccccagcgtagtctgggacgtcgtatggg

K112502

Construction of {<HA!} basic part K112502

Wobble PCR of cc001/cc003                  ( 63 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112502
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112503

Construction of {<myc>} basic part K112503

Wobble PCR of cc004/cc005                  ( 61 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112503
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005             Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG    

K112504

Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                  ( 64 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112504
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006             Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC