Template:Team:UC Berkeley/Notebook/CC construction

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(Difference between revisions)
(K112500)
(K112501)
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Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
-
Sub into pBca1256                          (EcoRI/BamHI)
+
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
Product is pBca1256-K112501
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Revision as of 20:20, 27 June 2008

Contents


K112500

Construction of {<HA>} basic part K112501

Wobble PCR of cc001/cc002                  ( 50 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910, L)
Product is pBca1256-K112500
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002             Reverse biobricking of {<HA>} 
ccagtggatccccagcgtagtctgggacgtcgtatggg

K112501

Construction of {<HA!} basic part K112502

Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112502

Construction of {<myc>} basic part K112503

Wobble PCR of cc004/cc005                  ( 51 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112502
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005             Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG    

K112503

Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                  ( 54 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI)
Product is pBca1256-K112503
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006             Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC