Template:Team:UC Berkeley/Notebook/CDB construction

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(Difference between revisions)
(New page: PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------...)
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PCR ca8373/ca8374 on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
PCR ca8373/ca8374 on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
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ca8374 Reverse Biobricking of mRFP
ca8374 Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc
CGttaGGATCCttactcccccaagaaaatcc
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Revision as of 06:25, 4 June 2008

PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc
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