Template:Team:UC Berkeley/Notebook/CDB construction

From 2008.igem.org

(Difference between revisions)
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grpE-R Reverse Biobricking    cgttaGGATCCcgcattcatctcgctaacttc
grpE-R Reverse Biobricking    cgttaGGATCCcgcattcatctcgctaacttc
 +
</pre>
 +
 +
==pBca1256-K112401==
 +
 +
<pre>
 +
Biobricking of promoter for recA protein on MG1655
 +
PCR recA-F and recA-R           (300 bp, EcoR1/BamH1/Dpn1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112401
 +
----------------------------
 +
recA-F Forward Biobricking    ccataGAATTCatgAGATCTagagaagcctgtcggcac
 +
recA-R Reverse Biobricking    cgttaGGATCCcgctttctgtttgtttt
 +
</pre>
 +
 +
==pBca1256-K112402==
 +
<pre>
 +
Biobricking of promoter for fabA protein on MG1655
 +
PCR fabA-F and fabA-R           (279 bp, EcoR1/BamH1/Dpn1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112402
 +
----------------------------
 +
fabA-F Forward Biobricking  ccataGAATTCatgAGATCTgccattacgttggctgaa
 +
fabA-R Reverse Biobricking  cgttaGGATCCccacccgtttcggtcatt
</pre>
</pre>

Revision as of 18:38, 27 June 2008

PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc

pBca1256-K112400

Biobricking of promoter for grpE protein on MG1655
 PCR grpE-F and grpE-R 	 	          (129 bp, EcoR1/BamH1/Dpn1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
grpE-F Forward Biobricking    ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga
grpE-R Reverse Biobricking    cgttaGGATCCcgcattcatctcgctaacttc

pBca1256-K112401

Biobricking of promoter for recA protein on MG1655
 PCR recA-F and recA-R 	 	          (300 bp, EcoR1/BamH1/Dpn1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
recA-F Forward Biobricking    ccataGAATTCatgAGATCTagagaagcctgtcggcac
recA-R Reverse Biobricking    cgttaGGATCCcgctttctgtttgtttt

pBca1256-K112402

Biobricking of promoter for fabA protein on MG1655
 PCR fabA-F and fabA-R 	 	          (279 bp, EcoR1/BamH1/Dpn1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
fabA-F Forward Biobricking   ccataGAATTCatgAGATCTgccattacgttggctgaa 
fabA-R Reverse Biobricking   cgttaGGATCCccacccgtttcggtcatt