Template:Team:UC Berkeley/Notebook/CDB construction

From 2008.igem.org

(Difference between revisions)
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<pre>
<pre>
-
Biobricking of promoter for grpE protein on MG1655
+
Biobricking of promoter for grpE protein  
-
  PCR grpE-F and grpE-R           (129 bp, EcoR1/BamH1/Dpn1)
+
  PCR grpE-F and grpE-R on MG1655   (129 bp, EcoRI/BamHI)
-
  Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
+
  Sub into pBca1256                   (EcoRI/BamHI 910+2472bp, L)
  Product is pBca1256-K112400
  Product is pBca1256-K112400
  ----------------------------
  ----------------------------
-
grpE-F Forward Biobricking    ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga
+
grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
-
grpE-R Reverse Biobricking    cgttaGGATCCcgcattcatctcgctaacttc
+
grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc
-
 
+
</pre>
</pre>
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<pre>
<pre>
-
Biobricking of promoter for recA protein on MG1655
+
Biobricking of promoter for recA protein  
-
  PCR recA-F and recA-R           (300 bp, EcoR1/BamH1/Dpn1)
+
  PCR recA-F and recA-R on MG1655   (317 bp, EcoR1/BamH1)
  Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
  Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
  Product is pBca1256-K112401
  Product is pBca1256-K112401
  ----------------------------
  ----------------------------
-
recA-F Forward Biobricking    ccataGAATTCatgAGATCTagagaagcctgtcggcac
+
recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
-
recA-R Reverse Biobricking    cgttaGGATCCcgctttctgtttgtttt
+
recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc
</pre>
</pre>
==pBca1256-K112402==
==pBca1256-K112402==
<pre>
<pre>
-
Biobricking of promoter for fabA protein on MG1655
+
Biobricking of promoter for fabA protein  
-
  PCR fabA-F and fabA-R           (279 bp, EcoR1/BamH1/Dpn1)
+
  PCR fabA-F and fabA-R on MG1655   (285 bp, EcoR1/BamH1)
  Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
  Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
  Product is pBca1256-K112402
  Product is pBca1256-K112402
  ----------------------------
  ----------------------------
-
fabA-F Forward Biobricking  ccataGAATTCatgAGATCTgccattacgttggctgaa
+
fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac
-
fabA-R Reverse Biobricking  cgttaGGATCCccacccgtttcggtcatt
+
fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
</pre>
</pre>

Revision as of 21:39, 27 June 2008

Contents

PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg