Template:Team:UC Berkeley/Notebook/JCA construction
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JCAnderson (Talk | contribs) (New page: <pre> PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------...) |
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+ | <pre> | ||
+ | PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | ||
+ | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
+ | Product is pBca1256-K112800 | ||
+ | ----------------------------------------------- | ||
+ | ca8373 Forward Biobricking of mRFP | ||
+ | CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag | ||
+ | ca8374 Reverse Biobricking of mRFP | ||
+ | CGttaGGATCCttactcccccaagaaaatcc | ||
+ | </pre> | ||
+ | |||
<pre> | <pre> | ||
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) |
Latest revision as of 17:32, 3 June 2008
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc