Template:Team:UC Berkeley/Notebook/Molly construction

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(Molly's Construction Files)
(Molly's Construction Files)
 
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<pre>
<pre>
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Construction of {<barstar!} Biobrick Part K112231
+
Construction of {rbs.xis.int!} Biobrick Part K112234
-
PCR mea033/mea034 on Bacillus amyloliquefaciens gen.
+
PCR mea018/mea006 on pAH57         (1317 bp, EcoRI/BamHI/DpnI)
-
(301 bp, EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
-
Product is pBca1256-K112231
+
Product is pBca1256-K112234
----------------------------------------
----------------------------------------
-
mea033 Forward Biobricking of {<barstar!} cgataGAATTCatgAGATCTaaaaaagcagtcattaacgggg
+
mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
-
mea034 Reverse Biobricking of {<barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc
+
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
-
</pre>
+
-
 
+
-
<pre>
+
-
Construction of {rbs.barstar!} Biobrick Part K112232
+
-
PCR mea035/mea034 on Bacillus amyloliquefaciens gen.
+
-
(320 bp, EcoRI/BamHI)
+
-
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
+
-
Product is pBca1256-K112232
+
-
----------------------------------------
+
-
mea035 Forward Biobricking of {rbs.barstar!} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
+
-
mea034 Reverse Biobricking of {rbs.barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc
+
-
</pre>
+
-
 
+
-
<pre>
+
-
Construction of {<phoA>} Biobrick Part K112233
+
-
PCR mea035/mea034 on ??????? (1219 bp, EcoRI/BamHI)
+
-
Sub into pBca9145 (EcoRI/BamHI, 2057+696, L)
+
-
Product is pBca9145-K112233
+
-
----------------------------------------
+
-
mea036 Forward Biobricking of {<phoA>} cgataGAATTCatgAGATCTggagattctgaaataaccgccg
+
-
mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCcttcaggcccagcgccgc
+
</pre>
</pre>
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>

Latest revision as of 00:06, 4 August 2008

Molly's Construction Files

Molly's Notebook

Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57		(230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc
Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc
Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57		(224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57		(234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017	Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc
Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57		(249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc
Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57		(1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57		(1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57		(1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019	Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57		(1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020	Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655		(331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg
Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg
Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655		(335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021	Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655		(350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022	Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655		(316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
----------------------------------------
mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015	Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015	Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655		(320 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023	Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655		(337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024	Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ompT>} Biobrick Part K112225
Wobble PCR mea025/mea026 		(97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112225
----------------------------------------
mea025	Forward Biobricking of {ompT>}	cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {a~ompT} Biobrick Part K112226
Wobble PCR mea027/mea026 		(101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112226
----------------------------------------
mea027	Forward Biobricking of {a~ompT}	cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {rbs.ompT} Biobrick Part K112227
Wobble PCR mea028/mea026 		(114 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112227
----------------------------------------
mea028	Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {pelB>} Biobrick Part K112228
Wobble PCR mea029/mea030 		(97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112228
----------------------------------------
mea029	Forward Biobricking of {pelB>}	cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {a~pelB} Biobrick Part K112229
Wobble PCR mea031/mea030 		(101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112229
----------------------------------------
mea031	Forward Biobricking of {a~pelB}	cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {rbs.pelB} Biobrick Part K112230
Wobble PCR mea032/mea030 		(116 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112230
----------------------------------------
mea032	Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {rbs.xis.int!} Biobrick Part K112234
PCR mea018/mea006 on pAH57 	        (1317 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112234
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc


Molly's Notebook