Template:Team:UC Berkeley/Notebook/Molly sequencing

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(Difference between revisions)
(Molly's Sequencing Log)
(Molly's Sequencing Log)
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| 234-CK-2 || 08/05/08 || pBjh1601CK-K112234 || 2 || G00101 || G00101 site deleted in Kan part of vector, bad read || [[Team:UC Berkeley/Notebook/MEA/234-CK-2_G00101 | 234-CK-2_G00101]]
| 234-CK-2 || 08/05/08 || pBjh1601CK-K112234 || 2 || G00101 || G00101 site deleted in Kan part of vector, bad read || [[Team:UC Berkeley/Notebook/MEA/234-CK-2_G00101 | 234-CK-2_G00101]]
|-
|-
-
| 234-1256-1 || 08/08/08 || pBca1256-K112234 || 1 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MEA/234-1256-1_ca998 | 234-1256-1_ca998]]
+
| 234-1256-#3 || 08/08/08 || pBca1256-K112234 || 3 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MEA/234-1256-#3_ca998 | 234-1256-#3_ca998]]
|-
|-
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| 234-1256-1 || 08/08/08 || pBca1256-K112234 || 1 || G00101 || Perfect || [[Team:UC Berkeley/Notebook/MEA/234-1256-1_G00101 | 234-1256-1_G00101]]
+
| 234-1256-#3 || 08/08/08 || pBca1256-K112234 || 3 || G00101 || Perfect || [[Team:UC Berkeley/Notebook/MEA/234-1256-#3_G00101 | 234-1256-#3_G00101]]
|-
|-
| MEA037 || Date || Plasmid || Clone # || Oligo || Result || [[Team:UC Berkeley/Notebook/MEA/MEA037 | MEA037]]
| MEA037 || Date || Plasmid || Clone # || Oligo || Result || [[Team:UC Berkeley/Notebook/MEA/MEA037 | MEA037]]

Revision as of 19:41, 12 August 2008

Molly's Sequencing Log

Molly's Notebook

Read Date Plasmid Clone # Oligo Result File
MEA001 06/15/08 pBca1256-K112200 1 ca998 Bad read MEA001
MA001 06/18/08 pBca1256-K112200 2 ca998 Point mutation changing Asn to Lys MA001
MEA002 06/15/08 pBca1256-K112201 1 ca998 Point mutation changing Asn to Lys MEA002
MA002 06/18/08 pBca1256-K112201 2 ca998 Point mutation changing Asn to Lys MA002
MEA003 06/15/08 pBca1256-K112202 1 ca998 Perfect MEA003
MEA004 06/15/08 pBca1256-K112203 1 ca998 Perfect MEA004
MEA005 06/15/08 pBca1256-K112204 1 ca998 Point mutation changing Asn to Lys MEA005
MA003 06/18/08 pBca1256-K112204 2 ca998 Bad Read MA003
MEA006 06/15/08 pBca1256-K112207 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA006
MEA006 06/18/08 pBca1256-K112207 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA006
AL305 06/23/08 pBca1256-K112207 1 G00101 Bad reverse read, 2 templates AL305
MEA007 06/15/08 pBca1256-K112208 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA007
MEA007 06/18/08 pBca1256-K112208 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA007
MA212 06/23/08 pBca1256-K112208 1 G00101 Perfect read, can see silent mutation from AGG to AGA seen in forward read MA212
MEA008 06/15/08 pBca1256-K112209 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA008
MEA008 06/18/08 pBca1256-K112209 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA008
MA209 06/23/08 pBca1256-K112209 1 G00101 Perfect reverse read, able to see the silent mutation from AGG to AGA seen in forward read MA209
MEA009 06/15/08 pBca1256-K112210 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA009
MEA009 06/18/08 pBca1256-K112210 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA009
MA211 06/23/08 pBca1256-K112210 1 G00101 Bad reverse read, two templates present MA211
MEA010 06/15/08 pBca1256-K112211 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA010
MEA010 06/18/08 pBca1256-K112211 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA010
MA210 06/23/08 pBca1256-K112211 1 G00101 Perfect reverse read, can see silent mutation from AGG to AGA seen in forward read MA210
MEA011 06/15/08 pBca1256-K112212 1 ca998 Partial read (long part) with silent mutation from AGG to AGA MEA011
MEA011 06/18/08 pBca1256-K112212 1 G00101 Bad reverse read, but good part was perfect with silent mutation from AGG to AGA MEA011
MA208 06/23/08 pBca1256-K112212 1 G00101 Bad reverse read, two templates present MA208
MEA012 06/15/08 pBca1256-K112213 1 ca998 Perfect MEA012
MEA013 06/15/08 pBca1256-K112214 1 ca998 Perfect MEA013
MEA014 06/15/08 pBca1256-K112215 1 ca998 Perfect MEA014
MEA015 06/15/08 pBca1256-K112216 1 ca998 Bad read in the beginning, re-sequence & pick new colony MEA015
MEA015 06/18/08 pBca1256-K112216 1 ca998 This one was perfect except it shows a deletion of A in EcoRI site, but that's probably just a problem with the sequencing data MEA015
MA005 06/18/08 pBca1256-K112216 2 ca998 Perfect MA005
MEA016 06/15/08 pBca1256-K112217 1 ca998 Perfect match to K112218 MEA016
MEA016 06/18/08 pBca1256-K112217 1 ca998 Perfect match to K112218 MEA016
MEA017 06/15/08 pBca1256-K112218 1 ca998 Perfect match to K112217 MEA017
MEA017 06/18/08 pBca1256-K112218 1 ca998 Perfect match to K112217 MEA017
MEA219 06/20/08 pBca1256-K1122219 1 ca998 Perfect MEA219
MEA220 06/20/08 pBca1256-K112220 1 ca998 Perfect, but there's an additional BamHI site inserted after the first one... MEA220
MEA018 06/15/08 pBca1256-K112221 1 ca998 Perfect MEA018
MEA019 06/15/08 pBca1256-K112222 1 ca998 Perfect MEA019
MEA223 06/20/08 pBca1256-K112223 1 ca998 Perfect MEA223
MEA224 06/20/08 pBca1256-K112224 1 ca998 Perfect MEA224
MEA020 06/15/08 pBca1256-K112225 1 ca998 Perfect MEA020
MEA021 06/15/08 pBca1256-K112226 1 ca998 Perfect MEA021
MEA022 06/15/08 pBca1256-K112227 1 ca998 Perfect MEA022
MEA023 06/15/08 pBca1256-K112228 1 ca998 Perfect match to K112229 MEA023
MEA024 06/15/08 pBca1256-K112229 1 ca998 Bad read, re-sequence MEA024
MA004 06/18/08 pBca1256-K112229 2 ca998 Insertion of an A... pick a new colony MA004
DV5 06/20/08 pBca1256-K112229 3 ca998 Perfect match to K112230 (aside from a deletion of an A in the EcoRI site... probably just due to bad reading. Don't worry about it) DV5
MEA025 06/15/08 pBca1256-K112230 1 ca998 Perfect match to K112228 MEA025
MEA026 06/24/08 pBca1256-K112200 1 ca998 Perfect MEA026
MEA027 06/24/08 pBca1256-K112201 1 ca998 Fatal point mutation from GCG to GTG (Ala to Val) MEA027
MEA028 06/24/08 pBca1256-K112204 1 ca998 Mutation in a~ region from tgcg to tgtg, and fatal deletion of G. MEA028
MEA029 06/24/08 pBca1256-K112205 1 ca998 Perfect MEA029
MEA030 06/24/08 pBca1256-K112207 1 g00101 Perfect MEA030
MEA031 06/24/08 pBca1256-K112210 1 g00101 Perfect MEA031
MEA032 06/24/08 pBca1256-K112212 1 g00101 Perfect MEA032
MEA033 06/26/08 pBca1256-K112201 2 ca998 Perfect MEA033
MEA034 06/26/08 pBca1256-K112201 3 ca998 Deletion of GCGATGT MEA034
MEA035 06/26/08 pBca1256-K112204 2 ca998 Perfect MEA035
MEA036 06/26/08 pBca1256-K112204 3 ca998 Insertion of GCGATGT MEA036
Molly6 07/22/08 pBjh1601KA-K112208 2 ca998 Perfect Molly6_ca998
Molly6 07/22/08 pBjh1601KA-K112208 2 G00101 Perfect Molly6_G00101
Molly7 07/22/08 pBjh1601AC-K112230 2 ca998 Sequenced perfectly for a~pelB (K112229) Molly7
Molly13-2 07/23/08 pBjh1601KA-K112235 2 ca998 Perfect Molly13-2_ca998
Molly13-2 07/23/08 pBjh1601KA-K112235 2 G00101 Perfect Molly13-2_G00101
Molly15-1 07/23/08 pBjh1601AK-{rbs.ompT}{<int!} 1 ca998 Perfect Molly15-1
Molly18-5 07/23/08 pBjh1601CA-{rbs.phoApp}{<ihfB!} 5 ca998 Perfect, but then we realized the wrong part was made initially. Molly18-5
Molly17-5 07/24/08 pBjh1601KC-{rbs.lamB}{<ihfA!} 5 ca998 Perfect, but then we realized the wrong part was made initially. Molly17-5
ig9 07/25/08 pBjh1601AK-K112205 1 ca998 Perfect ig9
Molly2-2 07/25/08 pBjh1601CA-K112212 2 ca998 Perfect sequence to a~int! (K112211) Molly2-2
Molly#3 07/26/08 pBjh1601AK-K112236 3 ca998 Perfect, shows deletion in Kan part of vector Molly#3
234-CK-1 08/05/08 pBjh1601CK-K112234 1 ca998 Perfect 234-CK-1_ca998
234-CK-1 08/05/08 pBjh1601CK-K112234 1 G00101 G00101 site deleted in Kan part of vector, bad read 234-CK-1_G00101
234-CK-2 08/05/08 pBjh1601CK-K112234 2 ca998 Perfect 234-CK-2_ca998
234-CK-2 08/05/08 pBjh1601CK-K112234 2 G00101 G00101 site deleted in Kan part of vector, bad read 234-CK-2_G00101
234-1256-#3 08/08/08 pBca1256-K112234 3 ca998 Perfect 234-1256-#3_ca998
234-1256-#3 08/08/08 pBca1256-K112234 3 G00101 Perfect 234-1256-#3_G00101
MEA037 Date Plasmid Clone # Oligo Result MEA037
MEA038 Date Plasmid Clone # Oligo Result MEA038
MEA039 Date Plasmid Clone # Oligo Result MEA039
MEA040 Date Plasmid Clone # Oligo Result MEA040
MEA041 Date Plasmid Clone # Oligo Result MEA041
MEA042 Date Plasmid Clone # Oligo Result MEA042
MEA043 Date Plasmid Clone # Oligo Result MEA043
MEA044 Date Plasmid Clone # Oligo Result MEA044
MEA045 Date Plasmid Clone # Oligo Result MEA045
MEA046 Date Plasmid Clone # Oligo Result MEA046
MEA047 Date Plasmid Clone # Oligo Result MEA047
MEA048 Date Plasmid Clone # Oligo Result MEA048
MEA049 Date Plasmid Clone # Oligo Result MEA049
MEA050 Date Plasmid Clone # Oligo Result MEA050