Template:Team:UC Berkeley/Notebook/SC construction

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<span style="font-family: Century Gothic; font-size: 10pt">
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<div style="text-align: right;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Back to Sherine's Notebook]] </div>
 +
<div style="text-align: right;"> [[Team:UC_Berkeley/Notebook| To All Notebooks]] </div>
__TOC__
__TOC__
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Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
-
Sub into pBca1256                (EcoRI/BamHI, 2227+ 910)
+
Sub into pBca1256                (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112600
Product is pBca1256-K112600
----------------------------------------------------
----------------------------------------------------
Line 21: Line 24:
Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
-
Sub into pBca1256                (EcoRI/BamHI, 2227+ 910)
+
Sub into pBca1256                (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112601
Product is pBca1256-K112601
--------------------------------------------------
--------------------------------------------------
Line 35: Line 38:
Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
-
Sub into pBca1256                (EcoRI/BamHI, 2227+ 910)
+
Sub into pBca1256                (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112602
Product is pBca1256-K112602
---------------------------------------------------
---------------------------------------------------
Line 49: Line 52:
Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
-
Sub into pBca1256                (EcoRI/BamHI, 2227+ 910)
+
Sub into pBca1256                (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112603
Product is pBca1256-K112603
-----------------------------------------------------
-----------------------------------------------------
Line 72: Line 75:
</pre>
</pre>
-
== pBca1256-K112403 ==
+
== pBjh1601AC-K112403 ==
<pre>
<pre>
Construction of {PhoA>} basic part K112403
Construction of {PhoA>} basic part K112403
            
            
CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)                               
CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)                               
-
Sub into pBca1256                       (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601AC                       (EcoRI/BamHI, 3195+910 L)
-
Product is pBca1256-K112403                                             
+
Product is pBjh1601AC-K112403                                             
-------------------------------------------------------------
-------------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cb10007    Fwd biobricking of {prepro>}
Line 86: Line 89:
</pre>
</pre>
-
== pBca1256-K112404  ==
+
== pBjh1601AC-K112404  ==
<pre>
<pre>
Construction of {LamB>} basic part K112404   
Construction of {LamB>} basic part K112404   
            
            
PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)                                 
PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)                                 
-
Sub into pBca1256                        (EcoRI/BamHI, 2472+910, L)                                               
+
Sub into pBjh1601AC                    (EcoRI/BamHI, 3195+910, L)                                               
-
Product is pBca1256-K112404                                           
+
Product is pBjh1601AC-K112404                                           
-------------------------------------------------------------
-------------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cb10009    Fwd biobricking of {prepro>}
Line 100: Line 103:
</pre>
</pre>
-
== pBca1256-K112605 ==
+
== pBjh1601KC-K112605 ==
<pre>
<pre>
Construction of {a~PhoA>} basic part K112605     
Construction of {a~PhoA>} basic part K112605     
          
          
PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                               
PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                               
-
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
-
Product is pBca1256-K112605
+
Product is pBjh1601KC-K112605
------------------------------------------------------
------------------------------------------------------
sc008      Fwd biobricking of {a~prepro>}
sc008      Fwd biobricking of {a~prepro>}
Line 114: Line 117:
</pre>
</pre>
-
== pBca1256-K112606 ==
+
== pBjh1601CK-K112606 ==
<pre>
<pre>
Construction of {a~LamB>} basic part K112606  
Construction of {a~LamB>} basic part K112606  
                  
                  
PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)                               
PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)                               
-
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601CK                             (EcoRI/BamHI, 3134+910, L)
-
Product is pBca1256- K112606
+
Product is pBjh1601CK-K112606
--------------------------------------------------
--------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
sc009      Fwd biobricking of {a~prepro>}
Line 128: Line 131:
</pre>
</pre>
-
== pBca1256-K112607  ==
+
== pBjh1601KC-K112607  ==
<pre>
<pre>
Construction of {rbs_PhoA>} basic part K112607               
Construction of {rbs_PhoA>} basic part K112607               
PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)                         
PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)                         
-
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
-
Product is pBca1256-K112607
+
Product is pBjh1601KC-K112607
------------------------------------------------------
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
sc010      Fwd biobricking of {rbs_prepro>}
Line 142: Line 145:
</pre>
</pre>
-
== pBca1256-K112608 ==
+
== pBjh1601KC-K112608 ==
<pre>
<pre>
Construction of {rbs_LamB>} basic part K112608   
Construction of {rbs_LamB>} basic part K112608   
        
        
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)             
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)             
-
Sub into pBca1256                                       (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601KC                                       (EcoRI/BamHI, 3132+910, L)
-
Product is pBca1256-K112608
+
Product is pBjh1601KC-K112608
------------------------------------------------------
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
sc011      Fwd biobricking of {rbs_prepro>}
Line 156: Line 159:
</pre>
</pre>
-
== pBca1256-K112609 ==
+
== pBjh1601CA-K112609 ==
<pre>
<pre>
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
          
          
PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                               
PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                               
-
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+
Sub into pBjh1601CA                             (EcoRI/BamHI, 2472+910, L)
-
Product is pBca1256-K112605
+
Product is pBjh1601CA-K112605
------------------------------------------------------
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
sc012      Fwd biobricking of {a~prepro>}
Line 175: Line 178:
== pBca1256-K112610 ==
== pBca1256-K112610 ==
<pre>
<pre>
-
Construction of {PhoA>} basic part  
+
Construction of {PhoA>} basic part K112610
          
          
-
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                               
+
PCR cb10007/sc013 on MG1655                   (94 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
-
Product is pBca1256-K112605
+
Product is pBca1256-K112610
------------------------------------------------------
------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cb10007    Fwd biobricking of {prepro>}
Line 189: Line 192:
== pBca1256-K112611 ==
== pBca1256-K112611 ==
<pre>
<pre>
-
Construction of {LamB>} basic part  
+
Construction of {LamB>} basic part K112611
          
          
-
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                               
+
PCR cb10009/sc014 on MG1655                   (107 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
Product is pBca1256-K112605
Line 198: Line 201:
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014    Reverse biobricking of {prepro>}
sc014    Reverse biobricking of {prepro>}
-
cgtagGGATCCcagccattgcctgagcagacattac                               
+
ccagtGGATCCagccattgcctgagcagac                               
</pre>
</pre>
== pBca1256-K112612 ==
== pBca1256-K112612 ==
<pre>
<pre>
-
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
+
Construction of {a~PhoA>} basic part K112612
          
          
-
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                               
+
PCR sc012/sc013 on MG1655                    (98 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
Product is pBca1256-K112605
Line 211: Line 214:
sc012      Fwd biobricking of {a~prepro>}
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
-
cb10008   Reverse biobricking of {a~prepro>}
+
sc013   Reverse biobricking of {prepro>}
-
cgtagGGATCCgggcttttgtcacaggggtaaac                               
+
ccagtGGATCCggcttttgtcacaggggtaaac
</pre>
</pre>
== pBca1256-K112613 ==
== pBca1256-K112613 ==
<pre>
<pre>
-
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
+
Construction of {a~LamB>} basic part K112613
          
          
-
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                               
+
PCR sc009/sc014 on MG1655                    (111 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
Product is pBca1256-K112605
------------------------------------------------------
------------------------------------------------------
-
sc012     Fwd biobricking of {a~prepro>}
+
sc009     Fwd biobricking of {a~prepro>}
-
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
+
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
-
cb10008   Reverse biobricking of {a~prepro>}
+
sc014   Reverse biobricking of {prepro>}
-
cgtagGGATCCgggcttttgtcacaggggtaaac                               
+
ccagtGGATCCagccattgcctgagcagac                               
</pre>
</pre>
== pBca1256-K112614 ==
== pBca1256-K112614 ==
<pre>
<pre>
-
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
+
Construction of {rbs_PhoA>} basic part K112614
          
          
-
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                               
+
PCR sc010/sc013 on MG1655                    (112 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
Product is pBca1256-K112605
------------------------------------------------------
------------------------------------------------------
-
sc012     Fwd biobricking of {a~prepro>}
+
sc010     Fwd biobricking of {rbs_prepro>}
-
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
+
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
-
cb10008   Reverse biobricking of {a~prepro>}
+
sc013   Reverse biobricking of {prepro>}
-
cgtagGGATCCgggcttttgtcacaggggtaaac                               
+
ccagtGGATCCggcttttgtcacaggggtaaac                             
</pre>
</pre>
== pBca1256-K112615 ==
== pBca1256-K112615 ==
<pre>
<pre>
-
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
+
Construction of {rbs_LamB>} basic part K112615
-
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                               
+
PCR sc011/sc014 on MG1655                    (126 bp, EcoRI/BamHI)                               
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
Product is pBca1256-K112605
------------------------------------------------------
------------------------------------------------------
-
sc012     Fwd biobricking of {a~prepro>}
+
sc011     Fwd biobricking of {rbs_prepro>}
-
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
+
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
-
cb10008   Reverse biobricking of {a~prepro>}
+
sc014   Reverse biobricking of {prepro>}
-
cgtagGGATCCgggcttttgtcacaggggtaaac                               
+
ccagtGGATCCagccattgcctgagcagac                             
</pre>
</pre>
-
 
+
== pBca1102-Bca9194 Assembly part ==
 +
<pre>
 +
PCR ca998/mea037 on Bca9194                          (1610 bp, EcoRI/BamHI?)
 +
Sub into {<tag!}.{b1006} composite part (CK vector)  (EcoRI/BamHI,____+__)
 +
Product is _______________
 +
---------------------------------------------------------
 +
ca998    Fwd biobricking
 +
gtatcacgaggcagaatttcag
 +
mea037    Reverse biobricking of {<phoA>}
 +
cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
 +
</pre>
----
----
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Sherine Cheung]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Sherine Cheung]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley Team Homepage]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley Team Homepage]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook| All Notebooks]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook| All Notebooks]] </div>

Latest revision as of 20:23, 8 August 2008

Back to Sherine's Notebook
To All Notebooks

Contents


pBca1256-K112600 

Construction of {<AP>} basic part K112600

Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601 

Construction of {<AP!} basic part K112601

Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001    Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003    Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602 

Construction of {<FLAG>} basic part K112602

Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112602
---------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603 

Construction of {<FLAG!} basic part K112603

Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112603
-----------------------------------------------------
SC004    Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006    Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604 

Construction of {rbs_barstar>} basic part K112604

PCR mea035/sc007 on Bacillus amyloliquefaciens gen.            (320 bp, EcoRI/BamHI)
Sub into pBca1256                                              (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035    Fwd biobricking of {rbs_barstar>} 
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg                              
sc007     Reverse biobricking of {rbs_barstar>}                     (Not needed...)
cgttaGGATCCagaaagtatgatggtgatgtcgc

pBjh1601AC-K112403 

Construction of {PhoA>} basic part K112403
          
CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)                              
Sub into pBjh1601AC                       (EcoRI/BamHI, 3195+910 L)
Product is pBjh1601AC-K112403                                            
-------------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601AC-K112404 

Construction of {LamB>} basic part K112404   
           
PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)                                 
Sub into pBjh1601AC                     (EcoRI/BamHI, 3195+910, L)                                               
Product is pBjh1601AC-K112404                                           
-------------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112605 

Construction of {a~PhoA>} basic part K112605    
         
PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112605
------------------------------------------------------
sc008      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601CK-K112606 

Construction of {a~LamB>} basic part K112606 
                
PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)                               
Sub into pBjh1601CK                             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112606
--------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010    Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112607 

Construction of {rbs_PhoA>} basic part K112607               

PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)                         
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112607
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601KC-K112608 

Construction of {rbs_LamB>} basic part K112608   
      
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)             
Sub into pBjh1601KC                                        (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112608
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601CA-K112609 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBjh1601CA                             (EcoRI/BamHI, 2472+910, L)
Product is pBjh1601CA-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


pBca1256-K112610 

Construction of {PhoA>} basic part K112610
         
PCR cb10007/sc013 on MG1655                   (94 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112610
------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112611 

Construction of {LamB>} basic part K112611 
         
PCR cb10009/sc014 on MG1655                   (107 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1256-K112612 

Construction of {a~PhoA>} basic part K112612 
         
PCR sc012/sc013 on MG1655                     (98 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112613 

Construction of {a~LamB>} basic part K112613
         
PCR sc009/sc014 on MG1655                     (111 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1256-K112614 

Construction of {rbs_PhoA>} basic part K112614
         
PCR sc010/sc013 on MG1655                     (112 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112615 

Construction of {rbs_LamB>} basic part K112615

PCR sc011/sc014 on MG1655                     (126 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1102-Bca9194 Assembly part 

PCR ca998/mea037 on Bca9194                           (1610 bp, EcoRI/BamHI?)
Sub into {<tag!}.{b1006} composite part (CK vector)   (EcoRI/BamHI,____+__) 
Product is _______________
---------------------------------------------------------
ca998     Fwd biobricking 
gtatcacgaggcagaatttcag
mea037    Reverse biobricking of {<phoA>}
cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
Sherine Cheung
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