Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
SC002R    Reverse Biobricking of <AP-tag>
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
-
 
</pre>
</pre>
Line 27: Line 26:
SC003    Reverse Biobricking of <AP-tag!
SC003    Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
-
 
</pre>
</pre>
Line 42: Line 40:
SC005    Reverse Biobricking of <FLAG-tag>
SC005    Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc
ccagtGGATCCcttgtcgtcgtcatccttgtagtc
-
 
</pre>
</pre>
== pBca1256-K112603 ==
== pBca1256-K112603 ==
<pre>
<pre>
-
 
Construction of <FLAG-tag! basic part K112603
Construction of <FLAG-tag! basic part K112603
Line 58: Line 54:
SC006  Reverse Biobricking of <FLAG-tag!
SC006  Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
-
 
</pre>
</pre>

Revision as of 18:55, 24 June 2008

Contents

pBca1256-K112600

Construction of <AP-tag> basic part K112600

Wobble PCR SC001/SC002  (66 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg


pBca1256-K112601

Construction of <AP-tag! basic part K112601

Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001     Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003     Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602

Construction of <FLAG-tag> basic part K112602

Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
-------------------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603

Construction of <FLAG-tag! basic part K112603

Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-------------------------------------------------------------
SC004   Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006   Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc