Template:Team:UC Berkeley/Notebook/SC construction

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pBca1256-K112600

Construction of <AP-tag> basic part K112600

Wobble PCR SC001/SC002  (66 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601

Construction of <AP-tag! basic part K112601

Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001     Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003     Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602

Construction of <FLAG-tag> basic part K112602

Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
-------------------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603

Construction of <FLAG-tag! basic part K112603

Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-------------------------------------------------------------
SC004   Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006   Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604

Construction of {rbs_barstar>} basic part K112604    (320 bp, EcoRI/BamHI)
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.   (EcoRI/BamHI, 2472+910, L)           
Sub into pBca1256                                             
Product is pBca1256-K112604
-------------------------------
sc007    Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc008    Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc  

pBca1256-K112605

Construction of {prepro>} of PhoA basic part K112605      (114 bp, EcoRI/BamHI)
CR sc009/sc010 on MG1655                                  (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112605
-------------------------------------------------------------
sc009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
sc010    Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


pBca1256-K112606

Construction of {prepro>} of LamB basic part K112606           (132 bp, EcoRI/BamHI)
PCR sc011/sc012 on MG1655                                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112606
-------------------------------------------------------------
sc011    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
sc012    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac


pBca1256-K112607

Construction of {a~prepro>} of PhoA basic part K112607                   (100 bp, EcoRI/BamHI)
PCR sc013/sc010                                                          (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112607
------------------------------------------------------
sc013    Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
sc010    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112608

Construction of {a~prepro>} of LamB basic part K112608           (111 bp, EcoRI/BamHI)
PCR sc014/sc012                                                  (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112608
--------------------------------------------------
sc014   Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taga atgatgattactctgc
sc012   Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112609

Construction of {rbs_prepro>} of PhoA basic part K112609               (113 bp, EcoRI/BamHI)
PCR sc015/sc010                                                        (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112609
------------------------------------------------------
sc015   Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT gtacatggagaaaataaaAtgaaacaaag
sc010   Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112610

Construction of {rbs_prepro>} of LamB basic part K112610                   (126 bp, EcoRI/BamHI)
PCR sc016/sc012                                                             (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112610
------------------------------------------------------
sc016 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT caatgactcaggagataga atgatgattac
sc012 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac



Sherine Cheung
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