Template:Team:UC Berkeley/Notebook/SC construction

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pBca1256-K112000

PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112000

Forward Biobricking of {T4 Holin!} ccagtGAATTCatgAGATCTatggcagcacctagaatatc Reverse Biobricking of {T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc