USTC/1 August 2008

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(Difference between revisions)
 
Line 8: Line 8:
*digest the biobrick BBa_B0034/32/30 with SpeI and pstI.
*digest the biobrick BBa_B0034/32/30 with SpeI and pstI.
*digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI.
*digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI.
-
*run gel and extract the digest DNA parts
+
*run gel and purify fragments from agarose gels
-
*ligate B0034 + C0070-B0015,B0032+C0070,B0032+C0070.
+
*ligate B0034 + C0070-B0015,B0032 + C0070,B0032 + C0070.

Latest revision as of 01:22, 28 October 2008

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31 July 2008 <|> 3 Agu 2008


Experiments

  • digest the biobrick BBa_B0034/32/30 with SpeI and pstI.
  • digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI.
  • run gel and purify fragments from agarose gels
  • ligate B0034 + C0070-B0015,B0032 + C0070,B0032 + C0070.
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