USTC/Notebook/DNA ligation

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DNA Ligation

DNA ligases are used with restriction enzymes to insert genes into plasmids.


Materials

  • DNA plasmids; . Measure concentration in Nanodrop beforehand.
  • Biobricks
  • equal amount of T4 ligase.


Procedure

The following volumes apply to a 20µl DNA ligation.

add the following components to PCR tube:

  • 9µl DNA plasmids
  • 1µl biobricks
  • 10ul T4 ligase
  • MIX the reaction by stiring
  • Incubate the reaction at 16*C for 2hrs or overnight to ensure complete ligation.
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