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USTC/Notebook/Restriction Digests
From 2008.igem.org
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'''[[Team:USTC/Notebook|< Back to Notebook]]''' | '''[[Team:USTC/Notebook|< Back to Notebook]]''' | ||
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*Appropriate NEB '''10x''' Buffer (check the NEB enzyme chart or catalogue to find compatible buffers). | *Appropriate NEB '''10x''' Buffer (check the NEB enzyme chart or catalogue to find compatible buffers). | ||
*Appropriate '''enzymes'''. | *Appropriate '''enzymes'''. | ||
| - | *'''ddH<sub> | + | *'''ddH<sub>2O''' |
*'''BSA''' (100x from NEB) | *'''BSA''' (100x from NEB) | ||
Revision as of 14:07, 27 October 2008
Restriction Digests
Materials
- DNA; the thing you want to cut. Usually plasmid or PCR product. Measure concentration in Nanodrop beforehand.
- Appropriate NEB 10x Buffer (check the NEB enzyme chart or catalogue to find compatible buffers).
- Appropriate enzymes.
- ddH2O
- BSA (100x from NEB)
