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User:Meagan/Assembly standards
From 2008.igem.org
People in community have developed various cloning techniques/assembly standards. iGEM supports three so far. (But there are a lot more being worked on)
Why would you want to use standard biological parts and an assembly standard?
- easier than traditional cloning techniques
- more reliable
- less time consuming
- separate function from assembly
- robotic assembly
BioBrick™ Assembly (TKnight)
BioBrick™ assembly is the original assembly standard created by Tom Knight. It was first described in his paper on Idempotent Vector Design for Standard Assembly of Biobricks. Since publication of that paper, this assembly standard has been adopted by Tom Knights lab the Registry of standard Biological Parts, iGEM, and many labs in the synthetic biology (based on standard biological parts) community.
Supported plasmids:
- pSBxxx series, e.g.:
- pSB1AC3
Restriction enzyme scheme:
- EcoRI (Prefix)
- XbaI (Prefix)
- SpeI (Suffix)
- PstI (Suffix)
