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User:University of Washington/1 July 2008
From 2008.igem.org
(Difference between revisions)
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- AraC sequences matched the biobrick (BBa_R0080). | - AraC sequences matched the biobrick (BBa_R0080). | ||
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| + | == BioBrick Promoter Measurements == | ||
| + | |||
| + | - All overnight cultures showed successful transformation by growing on plates with kanamycin. | ||
| + | |||
| + | - Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours. | ||
| + | |||
| + | - 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius. | ||
| + | |||
| + | - The remaining cultures were placed back into the 37 degree incubator to grow overnight. | ||
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[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 22:49, 1 July 2008
LuxR from AraC and TetR
- AraC sequences matched the biobrick (BBa_R0080).
BioBrick Promoter Measurements
- All overnight cultures showed successful transformation by growing on plates with kanamycin.
- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.
- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.
- The remaining cultures were placed back into the 37 degree incubator to grow overnight.
Team:University_of_Washington/Notebook#Notebook
