Wiki/Team:Warsaw/igem notebook.htm

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May
M	T	W	T	F	S	S
			[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_May_2008 1]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_May_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_May_2008 3]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_May_2008 4]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_May_2008 5]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_May_2008 7]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_May_2008 8]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_May_2008 10]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_May_2008 11]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_May_2008 12]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_May_2008 13]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_May_2008 14]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008 15]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_May_2008 17]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_May_2008 18]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008 20]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008 21]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008 22]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_May_2008 24]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_May_2008 25]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_May_2008 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008 28]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008 29]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_May_2008 30]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/31_May_2008 31]

June
M	T	W	T	F	S	S
						[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_June_2008 1]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008 3]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_June_2008 4]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_June_2008 5]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_June_2008 7]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_June_2008 8]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_June_2008 10]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008 11]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008 12]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008 13]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_June_2008 14]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_June_2008 15]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008 17]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_June_2008 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008 20]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008 21]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_June_2008 22]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008 24]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008 25]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_June_2008 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_June_2008 28]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_June_2008 29]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008 1]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008 3]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_July_2008 4]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008 5]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_July_2008 6]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_July_2008 7]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_July_2008 8]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_July_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_July_2008 10]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008 11]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008 12]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_July_2008 13]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008 14]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_July_2008 15]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_July_2008 17]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008 20]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_July_2008 21]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_July_2008 22]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_July_2008 24]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_July_2008 25]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_July_2008 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_July_2008 27]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_July_2008 28]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_July_2008 29]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_July_2008 30]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008 1]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_August_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008 4]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008 5]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_August_2008 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_August_2008 7]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_August_2008 8]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_August_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_August_2008 10]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008 11]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008 12]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008 13]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008 14]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_August_2008 15]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_August_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_August_2008 17]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_August_2008 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_August_2008 20]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008 21]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008 22]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_August_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_August_2008 24]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008 25]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_August_2008 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008 28]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008 29]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_August_2008 30]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/31_August_2008 31]

September
M	T	W	T	F	S	S
[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008 1]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008 3]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_September_2008 4]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008 5]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_September_2008 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_September_2008 7]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_September_2008 8]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_September_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_September_2008 10]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008 11]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_September_2008 12]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_September_2008 13]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_September_2008 14]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_September_2008 15]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008 17]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_September_2008 20]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_September_2008 21]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_September_2008 22]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_September_2008 24]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008 25]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_September_2008 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_September_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008 29]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008 30]

October
M	T	W	T	F	S	S
		[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_October_2008 1]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008 2]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008 3]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_October_2008 4]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_October_2008 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_October_2008 7]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_October_2008 8]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_October_2008 9]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008 10]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_October_2008 11]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_October_2008 12]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008 13]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_October_2008 14]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_October_2008 15]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008 16]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008 17]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_October_2008 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_October_2008 19]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_October_2008 20]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008 21]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008 22]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_October_2008 23]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/24_October_2008 24]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_October_2008 25]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_October_2008 26]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_October_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_October_2008 28]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_October_2008 29]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_October_2008 30]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/31_October_2008 31]

November
M	T	W	T	F	S	S
					[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 1]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 6]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_November_2008 7]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/16_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 18]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_November_2008 19]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_November_2008 20]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 26]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_November_2008 27]	[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_November_2008 28]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 30]

Our protocols

If not stated otherwise, all agarose gels bear Fermentas GeneRuler (100-10000 bp) ladder.

Our primers

Our vectors

Our strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10