Wisconsin: Lignin Project/16 July 2008
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+ | |'''Team Sorbitol'''<br> | ||
+ | Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.<br> | ||
+ | Start PCR srl operon from genomic DNA provided by Dr. Pfleger instead of MG1655. Still no band is shown on the gel.<br> | ||
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+ | '''Team Fungus'''<br> | ||
+ | Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).<br> | ||
+ | Started overnight cultures from transformation.<br> | ||
+ | Ran touchdown PCR using Taq using "good stuff" as template (correct band from 7/11).<br> | ||
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Latest revision as of 03:39, 29 October 2008
Team Sorbitol Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.
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