Wisconsin: Lignin Project/19 June 2008
From 2008.igem.org
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Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)<br> | Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)<br> | ||
In the meantime designed a digestion reaction to use once we get enough product.<br> | In the meantime designed a digestion reaction to use once we get enough product.<br> | ||
- | + | Ran model simulations of knockouts in program. Interesting knockout: | |
+ | TpiA~Triosephosphate isomerase | ||
+ | E.coli K12 MG1655, locus tag b3919, GeneID 948409, EC 5.3.1.1 | ||
+ | Obtained TpiA knockout from Keio Collection plated on LB+Kan | ||
+ | Determined best media formulation C Media supplemented with glycerol<br> | ||
'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Took spec reading of Carbon and Nitrogen-limited cDNA<br> | Took spec reading of Carbon and Nitrogen-limited cDNA<br> |
Latest revision as of 17:20, 18 October 2008
Team Sorbitol:
Purified the PCR product from yesterday - not enough DNA for a digestion |