Wisconsin: Lignin Project/24 June 2008

From 2008.igem.org

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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Sorbitol:'''
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Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.<br>
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Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.<br>
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We purified and stored this band at -20.<br>
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After discussions with faculty new C-Media recipe was obtained.<br>
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'''Team Fungus:''' <br>
'''Team Fungus:''' <br>
Ran leftovers from yesterday's PCR in 1% agrose gel<br>
Ran leftovers from yesterday's PCR in 1% agrose gel<br>
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Used QIAquick gel extraction microcentrifuge kit <br>
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-correct sized bands visible in UV light <br>
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Used QIAquick PCR purification<br>
+
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br>
-
<br>
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Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight<br>
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Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
+
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase <br>
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<br>
+
Used QIAquick PCR purification kit to purify PCR product <br>
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|}

Latest revision as of 19:43, 18 October 2008

Igemwibanner.gif
Team Sorbitol:

Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.
Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.
We purified and stored this band at -20.
After discussions with faculty new C-Media recipe was obtained.

Team Fungus:
Ran leftovers from yesterday's PCR in 1% agrose gel
-correct sized bands visible in UV light
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product
Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase
Used QIAquick PCR purification kit to purify PCR product