Wisconsin: Lignin Project/25 July 2008

From 2008.igem.org

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Digested the ''srlD'' and pET28a with BamHI and HindIII<br>
Digested the ''srlD'' and pET28a with BamHI and HindIII<br>
Extracted the digestion and performed a ligation which ran overnight at 4C<br>
Extracted the digestion and performed a ligation which ran overnight at 4C<br>
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'''Team Fungus:'''<br>
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Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br>
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Revision as of 16:44, 11 August 2008

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Team Sorbitol:

Digested the srlD and pET28a with BamHI and HindIII
Extracted the digestion and performed a ligation which ran overnight at 4C
Team Fungus:
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.