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Wisconsin: Lignin Project/25 July 2008
From 2008.igem.org
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Digested the ''srlD'' and pET28a with BamHI and HindIII<br> | Digested the ''srlD'' and pET28a with BamHI and HindIII<br> | ||
Extracted the digestion and performed a ligation which ran overnight at 4C<br> | Extracted the digestion and performed a ligation which ran overnight at 4C<br> | ||
| + | Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel<br> | ||
'''Team Fungus:'''<br> | '''Team Fungus:'''<br> | ||
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br> | Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br> | ||
Revision as of 20:15, 18 October 2008
| Team Sorbitol: Digested the srlD and pET28a with BamHI and HindIII
|
