Wisconsin: Lignin Project/25 July 2008

From 2008.igem.org

(Difference between revisions)
Line 8: Line 8:
Extracted the digestion and performed a ligation which ran overnight at 4C<br>
Extracted the digestion and performed a ligation which ran overnight at 4C<br>
Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel<br>
Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel<br>
 +
Digestion and Ligations performed. Success! <br>
'''Team Fungus:'''<br>
'''Team Fungus:'''<br>
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br>
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br>

Revision as of 20:16, 18 October 2008

Igemwibanner.gif
Team Sorbitol:

Digested the srlD and pET28a with BamHI and HindIII
Extracted the digestion and performed a ligation which ran overnight at 4C
Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel
Digestion and Ligations performed. Success!
Team Fungus:
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.