Wisconsin: Lignin Project/30 June 2008
From 2008.igem.org
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These products were purified and stored at 4C overnight.<br> | These products were purified and stored at 4C overnight.<br> | ||
Some of the product was used to start a second PCR reaction to run overnight.<br> | Some of the product was used to start a second PCR reaction to run overnight.<br> | ||
+ | Gowth curves using revised Cmedia with additional components completed to determine optimal growth media.<br> | ||
+ | <br> | ||
+ | '''Team Fungus:''' <br> | ||
+ | Grew BL21 pET28a plates for colonies 1 & 2, streaked out new plates, and selected colonies and grew them in liquid media over the weekend.<br> | ||
+ | Minipreped to purify DNA from colonies and stored them on ice.<br> | ||
+ | Ran a second PCR to test results and get more colonies, however electrophoresis returned inconclusive results.<br> | ||
+ | Digested purified plasmid and ran out on 1% agarose gel. Colonies 1a and 1b possibly have insert.<br> | ||
+ | Ran PCR using previous PCR products as template. Ran out on 1% agarose gel. Saw faint bands. <br> | ||
+ | Ran a PCR using purified plasmids as templates.<br> | ||
+ | Made LB broth.<br> | ||
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Latest revision as of 02:02, 29 October 2008
Team Sorbitol:
Repeated the successful PCR reaction from before. |