Wisconsin: Lignin Project/31 July 2008

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Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>
Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>
Used the QIAquick gel extraction kit to purify the double digested vector band.  Spec reading of 14 ng/uL.<br>
Used the QIAquick gel extraction kit to purify the double digested vector band.  Spec reading of 14 ng/uL.<br>
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Ran overnight ligation at 4 degrees C.
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Revision as of 17:26, 11 August 2008

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Team Fungus:

Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size.
Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.
Ran overnight ligation at 4 degrees C.