Wisconsin: Lignin Project/3 July 2008

From 2008.igem.org

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To do this we followed the protocol of the Weibel Lab.<br>
To do this we followed the protocol of the Weibel Lab.<br>
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C<br>
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C<br>
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further growth curves completed with C-media and TpiA knockout strain JW3890.<br>
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Revision as of 19:53, 18 October 2008

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Team Sorbitol:

Created SOC media for transformations.
Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a.
To do this we followed the protocol of the Weibel Lab.
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C
further growth curves completed with C-media and TpiA knockout strain JW3890.