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Home >> Wet Lab >> Protocols >> Making Overnight Cultures from Frozen Glycerol Cell Stocks

Preparing overnight cultures

Pippette 10mL LB into a red-capped culture tube or conical shake flask.
Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.)
Incubate at 37°C overnight.