Newcastle University Wetlab/4 August 2008

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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.

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September
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Monday 4th August

  • All 5 of us (Megan, Mark, Nina, Ria and Jess) went into the lab today to decide on a plan of action for the weeks to come.
  • We used Agarose Gel Electrophoresis to check for the presence of plasmids (pGFPrrnB - our plasmid with the gfp gene, and pJWV021 - with the mCherry gene). Our gel was inconclusive, so we decided to re-run it the following day to confirm our results.
  • We followed the extraction method outlined in the green folder and for each DNA spot we plated two agar dishes: one with a larger volume of DNA (4μl) and one with a smaller volume (2μl) to make colony counting easier. (See Making Agar Plates.) We plated the following and incubated at 37˚C overnight:

Plate 1: +ve control (isolated plasmid plus TOP10 cells)

Plate 2: -ve control (TOP10 cells only, no plasmid)

Plate 3: Large 1010 4A

Plate 4: Small 1010 4A

Plate 5: Large 1018 7A

Plate 6: Small 1018 7A