Protocols Electrophoresis

From 2008.igem.org

Making Gel

1% gel by dilution
=> 0.6g Low melt PCR agarose
=> 1.5mL 40x TAE
=> 58.5mL ddH2O ==> 1.5ul Ethidium Bromide

Running Gel

20ul digestion product
Add 5ul of 5x loading buffer
Load lane
10ul ladder marker

Run gel for 30-45 minutes

Protocols