Team:Chiba/Calendar-Home/7 September 2008

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6 September 2008 <|> 8 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
Sample No. 1,2
Sample DNA(μL) 12
PstI(μL) 0.5
SpeI(μL) 0.5
Buffer 2(μL) 2
(×10)BSA(μL) 2
dH2O(μL) 3
TOTAL(μL) 20


--->Gel Check
Chiba-0907.JPG
Sample leftright
Sample DNA(μL) 55
Loading Dye(μL) 11
TOTAL(μL) 66

Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2

-->(8/9)

Team:Output

Cloning Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
Sample No. 123456
DNA(digested sample)(μL) 505454451454
SpeI(μL) 100010
PstI(μL) 0.50.50.50.50.50.5
XbaI(μL) 00.50.50.500.5
BSA(x10)(μL) 0.677627
Buffer2(μL) 6---2-
Buffer3(μL) -776-7
dH2O(μL) 1.91120.51
TOTAL(μL) 60μl70μl70μl60μl20μl70μl

-->left at for 3 hours at 37 degrees.

-->Gel Extraction

Sample No. 123456
DNA(digested sample)(μL) 6101010410
Loading Dye(μL) 222212
dH2O(μL) 4---12
TOTAL(μL) 12121212612
well 1077657

-->DNA purification (1~4,6:7μl 5:6μl)