Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

Protocols

E.Coli

Preparation of competent cells (Barbara's Protocol)
[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)]
Transformation of competent cells (Barbara's Protocol)
Heat shock transformation

Cloning

Solutions and buffers

[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells]
[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes]
TENS for the do it yourself MiniPrep

Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]

Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/Protocols"