"

 

From 2008.igem.org

Jason

I performed a mini-prep of pUC57-ER, subsequently digesting it with XbaI and PstI, gel purifying the fragment and using it one of the following ligations.

I set up ligations for of pSB1A3+ER, pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB and left them overnight at 16 degrees C.

i was awesome today.

David

yesterday's transformations didn't work. not at all. no colonies. what is up with that?

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