Team:Michigan/Notebook/GelProtocol

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== <font color=royalblue size=5> Analytical Gel Standard Protocol </font> ==

1. Mix 5 &mu;L of DNA with 1 &mu;L of loading dye <br>
2. Pour heated liquid gel (with stoppers and combs in place) according to the number of samples you have to run, and the type of gel electrophoresis apparatus you have <br>
3. Allow gel to cool and solidify <br>
4. Remove all stoppers and combs <br>
5. Fill gel box with TAE until it completely covers the gel <br>
6. Load 3-4 &mu;L of dye/DNA mixture <br>
7. Load ladders for comparison <br>
8. Turn on current to ~45 V <br>
9. Once DNA enters gel, turn up current to ~100 V <br>
10. Allow gel to run until dye is ~3/4 of the way across the gel, depending on the size of your DNA <br>


== <font color=royalblue size=5> Gel Extraction Standard Protocol </font> ==


All of our gel extractions are performed using QIAGEN kits and protocols. 


QIAGEN's website can be found [http://www1.qiagen.com/ HERE].


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