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Create the page "Standard Media" on this wiki!

• Team:UCSF/Materials and Methods
  ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]). ...ere grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then treated with 1 μM α-factor (Zymo Research) to activate the pathw
  3 KB (499 words) - 03:27, 30 October 2008
• Team:Alberta NINT/Project
  [[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).
  21 KB (3,119 words) - 16:44, 30 March 2009
• Team:Hawaii/Member Training
  ## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds
  2 KB (309 words) - 12:16, 31 May 2008
• Team:UC Berkeley/Protocols
  # Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.
  17 KB (2,857 words) - 22:03, 27 October 2008
• Jamboree/Compete
  ...mboree/Compete#Requests_for_variance">Submit any requests for non-BioBrick standard methods</a> : <span class="deadline">October 8</span></li> ...xpected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also dec
  13 KB (1,920 words) - 06:14, 22 April 2016
• Team:Waterloo/Notebook
  ...plication written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will ...hat it can construct sequences for composite parts automatically. Although Standard Assembly makes it easy to ligate parts, it is fairly easy to reverse the en
  3 KB (456 words) - 04:05, 30 October 2008
• SOC
  #Follow directions to make 1 liter of [[SOB]] media [[Category:Material]] [[Category:Standard Media]]
  546 B (73 words) - 02:24, 30 May 2008
• Team:Waterloo/Project
  ...nucleases during uninduced conditions. Cultures will initially be grown on media containing IPTG. Expression of the unstable cI repressor (C0051) will be dr ...me degradation construct will be integrated into the genome via a proposed standard integration vector.
  11 KB (1,711 words) - 04:00, 30 October 2008
• Team:Groningen/Notebook
  ...e [http://partsregistry.org/Assembly:Standard_assembly <font color=#dd0000>Standard Assembly from the parts registry</font color>] site. When we had to assembl '''Media'''
  4 KB (643 words) - 12:10, 16 September 2008
• Alberta NINT/23 July 2008
  showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures
  968 B (108 words) - 23:02, 11 October 2008
• Team:Davidson-Missouri Western
  ...rts. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick, and other web-based tools to improve t i'''GEM 2008 powerpoint presentation''' [[Media:MWSU_Davidson_iGEM08.PPT]]
  4 KB (583 words) - 03:55, 30 October 2008
• Team:University of Washington/Measurement Kit
  ... After 16 hours, each overnight culture was diluted 100 fold into fresh M9 media with kanamycin, and grown for 3 hours. After three hours, two 200uL samples
  4 KB (577 words) - 04:09, 30 October 2008
• Team:Peking University/Project
  ...s yeast codon optimized kindly offered by Prof. Youri I Pavlov. A set of [[Media:Linkers.pdf|12 linkers]] were synthesized to test the ability to maintain t ...flanking the 3’ of the promoter. Other constructs were accomplished with standard protocols.
  18 KB (2,815 words) - 05:26, 30 October 2008
• Team:Heidelberg/Notebook/Killing II/5thweek
  *Retransformation of ligation into TOP 10 -> standard protocoll *Preparation of M9 media
  27 KB (3,683 words) - 21:27, 28 October 2008
• Brown: Team Resistance/14 July 2008
  *Lyse cells in minimal media instead of LB because the ion content of minimal media is controlled and known *use standard copper wire in the breadboard for the circuit and only use gold/platinum fo
  5 KB (771 words) - 18:58, 29 October 2008
• Teachtheteachers
  <b>10:00 am -- Jason Kelly -- Standard Measurement Kit for BioBrick Promoters (PoPS-out devices)</b><br> ...xplains how to measure the PoPs of promoters and compare it to a reference standard.
  5 KB (778 words) - 20:34, 8 May 2008
• Team:ETH Zurich/Project/Motivation
  ...ard E. coli, the cells with reduced genome grew slightly denser in certain media, tolerated a greater variety of plasmids and had higher electroporation eff
  6 KB (907 words) - 09:57, 30 October 2008
• Team:Caltech/Protocols/Measuring H2O2
  ...(100uM, 33.3uM, 11.1 uM, 3.7 uM and 0.0 uM H2O2) made in the corresponding media. [[Image:H2O2 standards example.png|thumb|center|400px|An example of a typical standard curve generated by the assay. Error bars (1SD, n=3) are too small to be see
  1 KB (162 words) - 00:24, 30 October 2008
• SOB
  [[Category:Material]] [[Category:Standard Media]]
  1 KB (171 words) - 02:13, 31 May 2008
• Team:Illinois/Antibody RTK Fusion
  ...pplies needed will include the cholera B subunit, yeast culture media, and standard molecular biology reagents/kits.
  7 KB (1,026 words) - 04:36, 30 October 2008