Team:Bologna/Parts
From 2008.igem.org
(Difference between revisions)
(→Composites) |
|||
(44 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
<html> | <html> | ||
<style type="text/css"> | <style type="text/css"> | ||
- | + | ||
body {background-color:#000000} | body {background-color:#000000} | ||
strong {color: #c09a6d;} | strong {color: #c09a6d;} | ||
Line 11: | Line 11: | ||
[[Image:Logo1a.gif|220px]][[Image:Testata_dx.jpg|745px]] | [[Image:Logo1a.gif|220px]][[Image:Testata_dx.jpg|745px]] | ||
!align="center"|[[Team:Bologna|HOME]] | !align="center"|[[Team:Bologna|HOME]] | ||
+ | !align="center"|[[Team:Bologna/Project|PROJECT]] | ||
!align="center"|[[Team:Bologna/Team|TEAM]] | !align="center"|[[Team:Bologna/Team|TEAM]] | ||
- | !align="center"|[[Team:Bologna/ | + | !align="center"|[[Team:Bologna/Software|SOFTWARE]] |
!align="center"|[[Team:Bologna/Modeling|MODELING]] | !align="center"|[[Team:Bologna/Modeling|MODELING]] | ||
- | !align="center"|[[Team:Bologna/ | + | !align="center"|[[Team:Bologna/Wetlab|WET LAB]] |
- | !align="center"|[[Team:Bologna/ | + | !align="center"|[[Team:Bologna/Notebook|LAB-BOOK]] |
!align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]] | !align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]] | ||
!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]] | !align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]] | ||
- | |||
|} | |} | ||
Line 26: | Line 26: | ||
= Composites = | = Composites = | ||
- | The table below shows all the composites that | + | The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find: |
- | - | + | -the link to the Registry in the second column ; |
- | -in the third column | + | -part components, in the third column ; |
-in the fourth column the plasmid the part has been cloned in; | -in the fourth column the plasmid the part has been cloned in; | ||
Line 43: | Line 43: | ||
{| | {| | ||
|- style="background: #c09a6d; text-align: center;" | |- style="background: #c09a6d; text-align: center;" | ||
- | |width= | + | |width=35| '''N°''' |
- | |width= | + | |width=85| '''Igem Code''' |
- | |width= | + | |width=540| '''Fragment''' |
|width=90| '''Plasmid''' | |width=90| '''Plasmid''' | ||
- | |width= | + | |width=90| '''Length (bp)''' |
- | |width= | + | |width=70| '''Vitality''' |
- | |width= | + | |width=30| '''Fluo''' |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>1</center> | | <center>1</center> | ||
- | | [http://partsregistry.org/Part:BBa_K079015 | + | | [http://partsregistry.org/Part:BBa_K079015 K079015] |
- | | [[Team:Bologna/Project#Concluding_the_iGEM_2007_Project|LacY transporter protein from E. coli]] | + | | [[Team:Bologna/Project#Concluding_the_iGEM_2007_Project|LacY transporter protein from E. coli (for the improvement of the registry)]] |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079015 1254] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079015 1254] | ||
Line 60: | Line 60: | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>2</center> | | <center>2</center> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
| [http://partsregistry.org/Part:BBa_K079017 K079017] | | [http://partsregistry.org/Part:BBa_K079017 K079017] | ||
| Lac symmetric - operator library member | | Lac symmetric - operator library member | ||
Line 83: | Line 67: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>3</center> |
| [http://partsregistry.org/Part:BBa_K079018 K079018] | | [http://partsregistry.org/Part:BBa_K079018 K079018] | ||
| Lac 1 - operator library member | | Lac 1 - operator library member | ||
Line 91: | Line 75: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>4</center> |
| [http://partsregistry.org/Part:BBa_K079019 K079019] | | [http://partsregistry.org/Part:BBa_K079019 K079019] | ||
| Lac 2 - operator library member | | Lac 2 - operator library member | ||
Line 99: | Line 83: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>5</center> |
| [http://partsregistry.org/Part:BBa_K079036 K079036] | | [http://partsregistry.org/Part:BBa_K079036 K079036] | ||
| Tet O operator library member | | Tet O operator library member | ||
Line 107: | Line 91: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>6</center> |
| [http://partsregistry.org/Part:BBa_K079037 K079037] | | [http://partsregistry.org/Part:BBa_K079037 K079037] | ||
| TetO-4C - operator library member | | TetO-4C - operator library member | ||
Line 115: | Line 99: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>7</center> |
| [http://partsregistry.org/Part:BBa_K079038 K079038] | | [http://partsregistry.org/Part:BBa_K079038 K079038] | ||
| TetO-wt/4C5G - operator library member | | TetO-wt/4C5G - operator library member | ||
Line 123: | Line 107: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>8</center> |
| [http://partsregistry.org/Part:BBa_K079039 K079039] | | [http://partsregistry.org/Part:BBa_K079039 K079039] | ||
| LexA 1 - operator library member | | LexA 1 - operator library member | ||
Line 131: | Line 115: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>9</center> |
| [http://partsregistry.org/Part:BBa_K079040 K079040] | | [http://partsregistry.org/Part:BBa_K079040 K079040] | ||
| LexA 2 - opeartor library member | | LexA 2 - opeartor library member | ||
Line 139: | Line 123: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>10</center> |
| [http://partsregistry.org/Part:BBa_K079041 K079041] | | [http://partsregistry.org/Part:BBa_K079041 K079041] | ||
| Lambda OR1 - operator library member | | Lambda OR1 - operator library member | ||
Line 147: | Line 131: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>11</center> |
| [http://partsregistry.org/Part:BBa_K079042 K079042] | | [http://partsregistry.org/Part:BBa_K079042 K079042] | ||
| Lambda OR2 - operator library member | | Lambda OR2 - operator library member | ||
Line 155: | Line 139: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>12</center> |
| [http://partsregistry.org/Part:BBa_K079043 K079043] | | [http://partsregistry.org/Part:BBa_K079043 K079043] | ||
| Lambda OR3 - operator library member | | Lambda OR3 - operator library member | ||
Line 163: | Line 147: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>13</center> |
| [http://partsregistry.org/Part:BBa_K079045 K079045] | | [http://partsregistry.org/Part:BBa_K079045 K079045] | ||
| Lac operator library | | Lac operator library | ||
Line 171: | Line 155: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>14</center> |
| [http://partsregistry.org/Part:BBa_K079046 K079046] | | [http://partsregistry.org/Part:BBa_K079046 K079046] | ||
| Tet operator library | | Tet operator library | ||
Line 179: | Line 163: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>15</center> |
| [http://partsregistry.org/Part:BBa_K079047 K079047] | | [http://partsregistry.org/Part:BBa_K079047 K079047] | ||
| Lambda operator library | | Lambda operator library | ||
Line 187: | Line 171: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>16</center> |
| [http://partsregistry.org/Part:BBa_K079048 K079048] | | [http://partsregistry.org/Part:BBa_K079048 K079048] | ||
| LexA operator library | | LexA operator library | ||
Line 194: | Line 178: | ||
| <center>Yes</center> | | <center>Yes</center> | ||
| <center>-</center> | | <center>-</center> | ||
+ | |- style="background:#f6efcd; color:black" | ||
+ | | <center>17</center> | ||
+ | | [http://partsregistry.org/Part:BBa_K079020 K079020] | ||
+ | | GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
+ | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079020 2165] | ||
+ | | <center>Yes</center> | ||
+ | | <center>[[Team:Bologna/Parts/FluoImages#K079020|►]]</center> | ||
+ | |- style="background:#f6efcd; color:black" | ||
+ | | <center>18</center> | ||
+ | | [http://partsregistry.org/Part:BBa_K079026 K079026] | ||
+ | | GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
+ | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079026 2116] | ||
+ | | <center>Yes</center> | ||
+ | | <center>[[Team:Bologna/Parts/FluoImages#K079026|►]]</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>19</center> | | <center>19</center> | ||
+ | | [http://partsregistry.org/Part:BBa_K079029 K079029] | ||
+ | | GFP expression controlled by LacI repressor under the BBa_J23114 promoter | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
+ | | 2501 | ||
+ | | <center>Yes</center> | ||
+ | | <center>[[Team:Bologna/Parts/FluoImages#K079029|►]]</center> | ||
+ | |- style="background:#f6efcd; color:black" | ||
+ | | <center>20</center> | ||
| [http://partsregistry.org/Part:BBa_K079049 K079049] | | [http://partsregistry.org/Part:BBa_K079049 K079049] | ||
| GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | | GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | ||
Line 203: | Line 211: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>21</center> |
| [http://partsregistry.org/Part:BBa_K079050 K079050] | | [http://partsregistry.org/Part:BBa_K079050 K079050] | ||
| GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | | GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | ||
Line 209: | Line 217: | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079050 981] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079050 981] | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | <center> | + | | <center>[[Team:Bologna/Parts/FluoImages#K079050|►]]</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>22</center> |
| [http://partsregistry.org/Part:BBa_K079023 K079023] | | [http://partsregistry.org/Part:BBa_K079023 K079023] | ||
- | | | + | | Auto-regulated J23118 constitutive promoter and Lac 2 operator |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079023 1243] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079023 1243] | ||
Line 219: | Line 227: | ||
| <center>-</center> | | <center>-</center> | ||
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
- | | <center> | + | | <center>23</center> |
- | | [http://partsregistry.org/Part: | + | | [http://partsregistry.org/Part:BBa_K079024 K079024] |
| LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator | | LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator | ||
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243] | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
| <center>Yes</center> | | <center>Yes</center> | ||
| <center>-</center> | | <center>-</center> | ||
Line 237: | Line 237: | ||
| <center>24</center> | | <center>24</center> | ||
| [http://partsregistry.org/Part:BBa_K079030 K079030] | | [http://partsregistry.org/Part:BBa_K079030 K079030] | ||
- | | GFP reporter protein | + | | GFP reporter protein under the control of the BBa_23105 constitutive promoter |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079030 957] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079030 957] | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | <center> | + | | <center>[[Team:Bologna/Parts/FluoImages#K079030|►]]</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>25</center> | | <center>25</center> | ||
Line 249: | Line 249: | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079031 957] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079031 957] | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | <center> | + | | <center>[[Team:Bologna/Parts/FluoImages#K079031|►]]</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>26</center> | | <center>26</center> | ||
Line 257: | Line 257: | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079032 957] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079032 957] | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | <center> | + | | <center>[[Team:Bologna/Parts/FluoImages#K079032|►]]</center> |
|} | |} | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
---- | ---- | ||
- | [https://2008.igem.org/Team:Bologna ''Up''] | + | [https://2008.igem.org/Team:Bologna/Parts ''Up''] |
Latest revision as of 02:18, 30 October 2008
HOME | PROJECT | TEAM | SOFTWARE | MODELING | WET LAB | LAB-BOOK | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
---|
Composites
The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:
-the link to the Registry in the second column ;
-part components, in the third column ;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| [http://partsregistry.org/Part:BBa_K079015 K079015] | LacY transporter protein from E. coli (for the improvement of the registry) | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] | 1254 | | |
| [http://partsregistry.org/Part:BBa_K079017 K079017] | Lac symmetric - operator library member | GENEART pGA18 | 20 | | |
| [http://partsregistry.org/Part:BBa_K079018 K079018] | Lac 1 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079019 K079019] | Lac 2 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079036 K079036] | Tet O operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079037 K079037] | TetO-4C - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079038 K079038] | TetO-wt/4C5G - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079039 K079039] | LexA 1 - operator library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079040 K079040] | LexA 2 - opeartor library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079041 K079041] | Lambda OR1 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079042 K079042] | Lambda OR2 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079043 K079043] | Lambda OR3 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079045 K079045] | Lac operator library | GENEART pGA18 | 78 | | |
| [http://partsregistry.org/Part:BBa_K079046 K079046] | Tet operator library | GENEART pMA | 61 | | |
| [http://partsregistry.org/Part:BBa_K079047 K079047] | Lambda operator library | GENEART pMA | 67 | | |
| [http://partsregistry.org/Part:BBa_K079048 K079048] | LexA operator library | GENEART pMA | 40 | | |
| [http://partsregistry.org/Part:BBa_K079020 K079020] | GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2165 | | |
| [http://partsregistry.org/Part:BBa_K079026 K079026] | GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2116 | | |
| [http://partsregistry.org/Part:BBa_K079029 K079029] | GFP expression controlled by LacI repressor under the BBa_J23114 promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2501 | | |
| [http://partsregistry.org/Part:BBa_K079049 K079049] | GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079050 K079050] | GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079023 K079023] | Auto-regulated J23118 constitutive promoter and Lac 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079024 K079024] | LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079030 K079030] | GFP reporter protein under the control of the BBa_23105 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079031 K079031] | GFP reporter protein under the control of the BBa_J23118 promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079032 K079032] | GFP reporter protein under the control of the BBa_J23100 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |