Team:Bologna

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<!---|<div style="font-size:162%; border:none; margin:0; padding:.1em; color:#000;">Igem 2008 Bologna</div>--->
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<center>[[Image:Example_logo.png|200px|center|Logo Bologna]]<br />IGEM 2008 BOLOGNA</center><br /></div>
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{{Team:Bologna/finestra|col1=#e4d2ba|col2=#ffffff|col3=#000000|titolo=<big>Portale {{PAGENAME}}</big>|contenuto=
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L<nowiki>'</nowiki>'''hip hop''' è un [[movimenti culturali|movimento culturale]] nato in prevalenza nelle comunità afroamericane e latine del [[Bronx]], sobborgo di [[New York]] nei primi [[anni 1970]]. Porzioni di tale cultura diventarono popolari ed uscirono dal loro iniziale confinio durante l'inizio degli [[anni 1980]], e negli [[anni 1990]] la cultura hip hop ha varcato i confini americani spandendosi un po' in tutto il mondo. Il movimento ha probabilmente mosso i primi passi con il lavoro di [[Kool DJ Herc|DJ Kool Herc]] che, competendo con DJ [[Afrika Bambaataa]] si dice abbia inventato il termine "hip hop" per descrivere la propria cultura.
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[[Image:Logo1a.gif|220px]][[Image:Testata_dx.jpg|745px]]
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!align="center"|[[Team:Bologna|HOME]]
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!align="center"|[[Team:Bologna/Project|PROJECT]]
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!align="center"|[[Team:Bologna/Team|TEAM]]
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!align="center"|[[Team:Bologna/Software|SOFTWARE]]
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!align="center"|[[Team:Bologna/Modeling|MODELING]]
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!align="center"|[[Team:Bologna/Wetlab|WET LAB]]
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!align="center"|[[Team:Bologna/Notebook|LAB-BOOK]]
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!align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]]
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!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]]
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I quattro principali aspetti, o "elementi", della cultura hip hop sono:
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* lo ''MC'ing'', anche noto come musica [[rap]], introdotto dagli [[afroamericani]];
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* il ''DJ'ing'', introdotto dai [[Giamaica|giamaicani]];
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* il ''writing'', ovvero l'arte dei [[Graffiti Writing|graffiti]], introdotto dai [[Porto Rico|portoricani]]
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* la [[breakdance]], sviluppata dagli afroamericani e portoricani.
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Alcuni considerano il [[beatboxing]] il ''quinto elemento'' dell'hip hop; mentre altri preferiscono aggiungere tra i principali aspetti l'attivismo politico, la moda tipica, lo [[slang]] ed il [[double dutching]] (una particolare forma di salto della corda).
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|[[Image:main.jpg|Logo Bologna|900px|center]]               
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{{Bologna/finestra100
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|titolo=[[Team:Bologna/Project|PROJECT]]
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|contenuto=[[Image:Nome_Progetto2.png|550px|left|Nome Progetto]]
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Our project aims to design a bacterial reprogrammable binary memory (EPROM) with genetically engineered E.coli. To engineer bacteria we designed a genetic Flip-Flop formed by an epigenetic memory sensitive to IPTG -for the memory reset- and an UV-sensitive trigger to set the memory ON. We designed the Flip-Flop circuit by model-based analysis and computer simulation. The core elements are two mutually regulated promoters. Each of them is composed of a constitutive promoter and an independent operator sequence. Thus, transcriptional strength and repressor binding affinity can be independently fixed. Since operator sites are still lacking in the Registry as standard parts, we cloned operator sequences for LacI, TetR, Lambda and LexA repressors and established an experimental procedure to characterize them. These parts allow the rational design of regulated promoters and we expect them to be a benefit in many Synthetic Biology applications.
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[[Team:Bologna/Project|Click here for more information...]]
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[[Team:Bologna/finestra]]
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{{Bologna/finestra100
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|titolo=[[Team:Bologna/Team|TEAM]]
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|contenuto=[[Image:TeamBo2.JPG|250px|right]]
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The leading idea behind the UniBO Team was to put in touch students of the same University with different backgrounds to favor competence and skill sharing. Students from different Faculties (Biotechnology, Pharmacy and Engineering) answered to the iGEM call and formed the TEAM. They were moved by a common desire for new experiences and they decided to participate in this exciting competition to go deep in Synthetic Biology. The Team worked hard during the summer to become fully operative, overcoming differences both in training and personality.
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<br><br>
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[[Team:Bologna/Team|Go to the page!]]
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}}
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[[Image:TeamBo1.JPG|200px|thumbnail|our team]]
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{{Bologna/finestra100
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<!--- The Mission, Experiments --->
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|titolo=[[Team:Bologna/Modeling|MODELING]]
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|contenuto=[[Image:Circuito2.jpg|278px|right]]
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Mathematical model analysis and computer simulations were used to design the Flip-Flop circuit. We resorted to this engineer’s approach  to obtain a  rational design of regulated promoters, that are the core element of the circuit.  By the model we found the analytic relationships to quote the regulated promoter in terms of transcriptional strength and sensitivity to the repressor. By model-based computer simulation we established such a relevant circuit properties as the bistability and the dynamical response to inputs. A simple procedure to characterize the operator sequence was theoretically derived. [[Team:Bologna/Modeling|Enjoy yourself!]]
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{{Bologna/finestra100
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|titolo=[[Team:Bologna/Software|SOFTWARE]]
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|contenuto=[[Image:cuscino.JPG|260px|right]]
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Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this [[Team:Bologna/Software|page]].
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!align="center"|[[Team:Bologna|HOME]]
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{{Bologna/finestra100
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!align="center"|[[Team:Bologna/Team|THE TEAM]]
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|titolo=[[Team:Bologna/Wetlab|WET LAB]]
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!align="center"|[[Team:Bologna/Project|THE PROJECT]]
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!align="center"|[[Team:Bologna/Parts|PARTS SUBMITTED TO THE REGISTRY]]
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<br><br><br><br><br><br><br>
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!align="center"|[[Team:Bologna/Modeling|MODELING]]
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To obtain regulated promoters we synthesized four libraries of operator sequences, respectively for LacI, TetR, CI and LexA repressor proteins. In each library, there are three sequences each with a different repressor binding affinity. The choice of the operator should give a relative fine-tuning of promoter sensitivity to the repressor. In this part, it is explained how we designed the operator libraries and isolated single operators to clone them in the standard format. Moreover, we built and tested: a LacI regulated promoter to tune promoter activation and a lexA based promoter to study the SOS activation (UV and hydrogen peroxide). [[Team:Bologna/Wetlab|So wet!]]
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!align="center"|[[Team:Bologna/Notebook|NOTEBOOK]]
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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= Aknowledgements =
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* ''' [http://www.unibo.it/Portale/default.htm University of Bologna] '''
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<br>
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[[Image:AlmaMaterStudiorum.jpg|left|100 px]]
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<br><br><br><br><br><br><br>
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* ''' [http://serinar.criad.unibo.it Ser.In.Ar. Cesena] '''
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[[Image:Ser_In_Ar.jpg|left|500px]]
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<br><br><br><br><br>
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[https://2008.igem.org/Team:Bologna ''Up'']

Latest revision as of 02:39, 30 October 2008

Logo1a.gifTestata dx.jpg
HOME PROJECT TEAM SOFTWARE MODELING WET LAB LAB-BOOK SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Logo Bologna


PROJECT
Nome Progetto

Our project aims to design a bacterial reprogrammable binary memory (EPROM) with genetically engineered E.coli. To engineer bacteria we designed a genetic Flip-Flop formed by an epigenetic memory sensitive to IPTG -for the memory reset- and an UV-sensitive trigger to set the memory ON. We designed the Flip-Flop circuit by model-based analysis and computer simulation. The core elements are two mutually regulated promoters. Each of them is composed of a constitutive promoter and an independent operator sequence. Thus, transcriptional strength and repressor binding affinity can be independently fixed. Since operator sites are still lacking in the Registry as standard parts, we cloned operator sequences for LacI, TetR, Lambda and LexA repressors and established an experimental procedure to characterize them. These parts allow the rational design of regulated promoters and we expect them to be a benefit in many Synthetic Biology applications. Click here for more information...



TEAM
TeamBo2.JPG

The leading idea behind the UniBO Team was to put in touch students of the same University with different backgrounds to favor competence and skill sharing. Students from different Faculties (Biotechnology, Pharmacy and Engineering) answered to the iGEM call and formed the TEAM. They were moved by a common desire for new experiences and they decided to participate in this exciting competition to go deep in Synthetic Biology. The Team worked hard during the summer to become fully operative, overcoming differences both in training and personality.

Go to the page!


MODELING
Circuito2.jpg

Mathematical model analysis and computer simulations were used to design the Flip-Flop circuit. We resorted to this engineer’s approach to obtain a rational design of regulated promoters, that are the core element of the circuit. By the model we found the analytic relationships to quote the regulated promoter in terms of transcriptional strength and sensitivity to the repressor. By model-based computer simulation we established such a relevant circuit properties as the bistability and the dynamical response to inputs. A simple procedure to characterize the operator sequence was theoretically derived. Enjoy yourself!

SOFTWARE
Cuscino.JPG

Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this page.


WET LAB
Collage2.JPG








To obtain regulated promoters we synthesized four libraries of operator sequences, respectively for LacI, TetR, CI and LexA repressor proteins. In each library, there are three sequences each with a different repressor binding affinity. The choice of the operator should give a relative fine-tuning of promoter sensitivity to the repressor. In this part, it is explained how we designed the operator libraries and isolated single operators to clone them in the standard format. Moreover, we built and tested: a LacI regulated promoter to tune promoter activation and a lexA based promoter to study the SOS activation (UV and hydrogen peroxide). So wet!











































Aknowledgements

  • [http://www.unibo.it/Portale/default.htm University of Bologna]


AlmaMaterStudiorum.jpg








  • [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]


Ser In Ar.jpg








Up