Team:ETH Zurich
From 2008.igem.org
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- | + | [[Image:ETH_Logo.JPG|center|850px]] | |
== Project Abstract == | == Project Abstract == | ||
- | '''''' | + | ''''''Random walks towards the minimal genome''''''<br> |
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Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering. | Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering. | ||
We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is based on an iterative cycle of genome reduction and strain selection. | We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is based on an iterative cycle of genome reduction and strain selection. | ||
- | We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based | + | We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore, we develop a chemostat-based condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection."<br> |
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- | == | + | =="Meet us" video== |
+ | <html> | ||
+ | <center> | ||
+ | <script type="text/javascript" src="http://widgets.clearspring.com/o/46928cc51133af17/48e78de6aca507ba/46928cc5788deb29/3fdd2ef6/-cpid/9a26a079cd6c96e1/autostart/false/repeat/false/widget.js"></script> | ||
+ | </center> | ||
+ | </html> | ||
- | + | == The Team == | |
- | + | [[Image:Team_eth.JPG|left|thumb|150px|Team ETH]] | |
+ | The iGEM team 2008 follows the tradition of the ETH teams before, and consits of students from very diverse scientific backgrounds. | ||
+ | If you'd like to know more about the team, you will find more information in the [https://2008.igem.org/Team:ETH_Zurich/Team/Members Team Section].<br> | ||
+ | |||
+ | <strong>Undergraduate Students</strong><br> | ||
+ | Julia Slipantschuk, Sebastian Giese, Nico Hensgens, Rebecca Herbst, Reine Byun, [http://www.facebook.com/profile.php?id=804108753&ref=profile Georg Ofenbeck]. | ||
+ | |||
+ | <br><strong>Graduate Students</strong><br> | ||
+ | [https://2008.igem.org/User:snev Sven Lucas Dietz], [https://2007.igem.org/User:Luca.Gerosa Luca Gerosa], [http://www.biol.ethz.ch/IMB/groups/ban_group/members/jrabl Julius Rabl]. | ||
+ | |||
+ | <br><strong>Advisors</strong><br> | ||
+ | [http://www.ipe.ethz.ch/laboratories/bpl/people/panke Sven Panke], [http://www.ipe.ethz.ch/laboratories/bpl/people/meyer Andreas Jörg Meyer], [http://www.csb.ethz.ch/people/joergs Jörg Stelling]. | ||
+ | <br clear="all" /> | ||
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+ | == Achievements == | ||
+ | [[Image:Results_seal_gold.jpg]][[Image:Results_seal_silver.jpg]][[Image:Results_seal_bronze.jpg]] | ||
+ | For all the award relevant issues that had to be addressed, check out our [https://2008.igem.org/Team:ETH_Zurich/Project/Medal_Relevant Medal Relevant Section] in which we listed how we strived for our goals. | ||
+ | |||
+ | == Site Map == | ||
+ | <html><center></html> | ||
+ | {|style="width:90%; border=0;background-color: lightgrey;text-align: center;" | ||
! Home !! Team !! Project !! Wetlab !! Modeling !! Tools | ! Home !! Team !! Project !! Wetlab !! Modeling !! Tools | ||
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| [https://2008.igem.org/Team:ETH_Zurich/Project/Motivation Motivation] | | [https://2008.igem.org/Team:ETH_Zurich/Project/Motivation Motivation] | ||
| [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Genome_Reduction Genome Reduction] | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Genome_Reduction Genome Reduction] | ||
- | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/ | + | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Framework Framework] |
| [https://2008.igem.org/Team:ETH_Zurich/Tools/Automated_Wiki Automated Wiki] | | [https://2008.igem.org/Team:ETH_Zurich/Tools/Automated_Wiki Automated Wiki] | ||
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| [https://2008.igem.org/Team:ETH_Zurich/Project/Background Background] | | [https://2008.igem.org/Team:ETH_Zurich/Project/Background Background] | ||
| [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Chemostat_Selection Chemostat Selection] | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Chemostat_Selection Chemostat Selection] | ||
- | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Genome | + | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Genome_Static_Analysis Genome Restriction Enzyme Analysis] |
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| [https://2008.igem.org/Team:ETH_Zurich/Project/Applications Applications] | | [https://2008.igem.org/Team:ETH_Zurich/Project/Applications Applications] | ||
| [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Switch_Circuit Switch Circuit] | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Switch_Circuit Switch Circuit] | ||
- | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/ | + | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Genome-Scale_Model Genome Scale Model] |
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| [https://2008.igem.org/Team:ETH_Zurich/Project/Conclusions Conclusions] | | [https://2008.igem.org/Team:ETH_Zurich/Project/Conclusions Conclusions] | ||
- | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/ | + | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Submitted_Parts Submitted Parts] |
- | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/ | + | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Chemostat_Selection Chemostat Selection] |
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| [https://2008.igem.org/Team:ETH_Zurich/Project/Medal_Relevant Medal Relevant] | | [https://2008.igem.org/Team:ETH_Zurich/Project/Medal_Relevant Medal Relevant] | ||
- | | | + | | [https://2008.igem.org/Team:ETH_Zurich/Wetlab/Safety Lab Safety] |
- | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/ | + | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Switch_Circuit Switch Circuit] |
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| [https://2008.igem.org/Team:ETH_Zurich/Modeling/Download Download] | | [https://2008.igem.org/Team:ETH_Zurich/Modeling/Download Download] | ||
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Latest revision as of 03:14, 30 October 2008
Project Abstract'Random walks towards the minimal genome' "This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome.
Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering.
We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is based on an iterative cycle of genome reduction and strain selection.
We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore, we develop a chemostat-based condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection." "Meet us" video
The TeamThe iGEM team 2008 follows the tradition of the ETH teams before, and consits of students from very diverse scientific backgrounds.
If you'd like to know more about the team, you will find more information in the Team Section. Undergraduate Students
AchievementsFor all the award relevant issues that had to be addressed, check out our Medal Relevant Section in which we listed how we strived for our goals. Site Map
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