Team:Virginia

From 2008.igem.org

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<a href="https://2008.igem.org/Team:Virginia/Project"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/project.gif">Projects&nbsp;</a><span onClick="showHide('projects')" class=expander>&raquo;</span><br>
<a href="https://2008.igem.org/Team:Virginia/Project"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/project.gif">Projects&nbsp;</a><span onClick="showHide('projects')" class=expander>&raquo;</span><br>
<div id=projects class=hide>
<div id=projects class=hide>
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<a href="#">Transcription Attenuators</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Project#ga">Genetic Attenuators</a><br>
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<a href="#">Placeholder Sites</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Project#ph">BioBrick Placeholders</a><br>
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<a href="#">BioPlastic</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Project#bp">BioPlastic</a><br>
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<a href="#">Adding to the RSBP</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Project#rsbp">Adding to the RSBP</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Parts"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/parts.gif">BioBricks</a><br>
<a href="https://2008.igem.org/Team:Virginia/Parts"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/parts.gif">BioBricks</a><br>
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<a href="http://www4.clustrmaps.com/counter/maps.php?url=https://2008.igem.org/Team:Virginia" id="clustrMapsLink"><img class="logos" src="http://www4.clustrmaps.com/counter/index2.php?url=https://2008.igem.org/Team:Virginia" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /></a>
<a href="http://www4.clustrmaps.com/counter/maps.php?url=https://2008.igem.org/Team:Virginia" id="clustrMapsLink"><img class="logos" src="http://www4.clustrmaps.com/counter/index2.php?url=https://2008.igem.org/Team:Virginia" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /></a>
<br><span>We'd like to thank our generous sponsors for making our work possible:</span><br>
<br><span>We'd like to thank our generous sponsors for making our work possible:</span><br>
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<a href="http://www.virginia.edu/"><img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/uva-logo-patch.png" alt="University of Virginia" title="University of Virginia" /></a>
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<img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/uva-logo-patch.png" alt="University of Virginia" title="University of Virginia" /><img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/dupont.gif" alt="duPont" title="duPont" />
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<a href="http://dupont.com"><img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/dupont.gif" alt="duPont" title="duPont" /></a>
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</div>
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<h3>Genetic Attenuators</h3>
<h3>Genetic Attenuators</h3>
<p>Getting in control of transcription</p>
<p>Getting in control of transcription</p>
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<p>Terminators are never 100% efficient, meaning that not all polymerases will disengage from the strand they are operating on. This phenomenon can be exploited to create <i>Genetic Attentuators</i>. Inserting a <i>Genetic Attenuator</i> between genes will create transcripts containing different sets of genes. Varying the number of copies of a gene present in the transcript will influence downstream translation of that gene.
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<p>Terminators are never 100% efficient, meaning that not all polymerases will disengage from the strand they are operating on. This phenomenon can be exploited to create inefficient terminators we call <i>Genetic Attentuators</i>. Inserting a <i>Genetic Attenuator</i> between two genes will result in different levels of two transcripts. The levels of polycistronic mRNA will be lower than the levels of mRNA corresponding to only the upstream gene. How much lower depends on the efficiency of the attenuator.</p>
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</p>
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<a href="https://2008.igem.org/Team:Virginia/Project#ga"><img src="http://partsregistry.org/wiki/images/f/f4/Mfold-B0012-1.png" width=340 /></a>
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<span>MORE</span>
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<span>&nbsp;</span>
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</div>
<div class="box">
<div class="box">
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<h3>Placeholder Sites</h3>
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<h3>BioBrick Placeholders</h3>
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<p>Cloning sites made easy!</p>
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<p>A new technical standard!</p>
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<p>Assembling gene constructs is a tedious procedure that consumes valuable time. When assembling several BioBricks into a vector it would be valuable to be able to insert a cloning site which could later be used to insert a BioBrick into a certain part of the vector. <i>Placeholder Sites</i> accomplish this task by providing restriction sites compatible with the BioBrick standard inside of a standard part.</p>
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<p>Assembling composite BioBricks is a tedious procedure that consumes valuable time. When assembling several BioBricks, it would be nice to be able to insert a BioBrick Placeholder that could later be used to insert a BioBrick part. <i>BioBrick Placeholders</i> accomplish this task by providing internal restriction sites compatible with the standard BioBrick restriction sites.</p>
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<ol>
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<center><a href="https://2008.igem.org/Team:Virginia/Project#ph"><img src="http://partsregistry.org/wiki/images/f/ff/Bricks.png" style="margin-top:140px;margin-bottom: 5px;"></a></center>
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<li>EX - ApoI (NotI) AvrII - SP</li>
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<span>&nbsp;</span>
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<li>EX - ApoI (NotI) NheI - SP</li>
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<li>EX - ApoI (NotI) NsiI - SP</li>
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<li>EX - ApoI (NotI) SbfI - SP</li>
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<li>EX - MfeI (NotI) AvrII - SP</li>
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<li>EX - MfeI (NotI) NheI - SP</li>
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<li>EX - MfeI (NotI) Nsil - SP</li>
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<li>EX - MfeI (NotI) SbfI - SP</li>
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</ol>
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<span>MORE</span>
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<h3>BioPlastic</h3>
<h3>BioPlastic</h3>
<p>Growing a renewable resource</p>
<p>Growing a renewable resource</p>
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<p></p>
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<p>Polyhydroybutyrate (PHB) is a polykydroxyalkanoate (PHA), a polyester polymer that is naturally synthesized by certain microorganisms. There is enormous potential for PHB production as a plastic material due to the fact that it has similar physical properties of polypropylene, a ubiquitous thermoplastic polymer derived from petroleum. Furthermore, PHB is biologically degradable.</p>
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<img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/pathway-small.jpg" />
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<a href="https://2008.igem.org/Team:Virginia/Project#bp"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/pathway-small.jpg" style="margin-top:165px; margin-bottom: 5px;" /></a>
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<p></p>
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<span>&nbsp;</span>
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<span>MORE</span>
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</div>
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<h3>Additions to the Registry</h3>
<h3>Additions to the Registry</h3>
<p>Giving back</p>
<p>Giving back</p>
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<p></p>
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<p>In addition to adding our Genetic Attenuators, BioBrick Placeholders and bioplastic parts to the Registry, we have also submitted parts that code for orange fluorescent protein (OFP), strongly enhanced blue fluorescent protein (SBFP2) and streptomycin 3'-adenyltransferase, which enables resistance to the antibiotic streptomycin</p>
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<span>MORE</span>
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<a href="https://2008.igem.org/Team:Virginia/Project#rsbp"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/vgem-ofp.jpg" width=350 /></a>
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<span>&nbsp;</span>
</div>
</div>

Latest revision as of 03:53, 30 October 2008


Home
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We'd like to thank our generous sponsors for making our work possible:
University of VirginiaduPont

Transcription attenuation for metabolic control by engineering intrinsic terminators

A main challenge in constructing synthetic biological systems is the inability to precisely regulate gene expression using artificial means. Tightly-regulated control of any given set of related transcriptional, translational and posttranslational events will likely require a combination of powerful strategies. Therefore, the 2008 Virginia iGEM team is developing a library of transcriptional terminators intentionally redesigned to be functionally inefficient. Well-characterized, standardized terminators of various efficiencies should allow finely-tuned transcription attenuation and represents yet another step toward global biological control. This work complements other gene expression control methods that focus on initiation of transcription. The desired result is quantitative control of transcript levels, which is often necessary to balance flux through a synthetic metabolic pathway. To demonstrate its potential for real-world application, the team is planning to employ this approach to control the expression of a heterologous pathway in E. coli for the biosynthesis of polyhydroxybutyrate (PHB), a biodegradable polyester plastic.

Genetic Attenuators

Getting in control of transcription

Terminators are never 100% efficient, meaning that not all polymerases will disengage from the strand they are operating on. This phenomenon can be exploited to create inefficient terminators we call Genetic Attentuators. Inserting a Genetic Attenuator between two genes will result in different levels of two transcripts. The levels of polycistronic mRNA will be lower than the levels of mRNA corresponding to only the upstream gene. How much lower depends on the efficiency of the attenuator.

 

BioBrick Placeholders

A new technical standard!

Assembling composite BioBricks is a tedious procedure that consumes valuable time. When assembling several BioBricks, it would be nice to be able to insert a BioBrick Placeholder that could later be used to insert a BioBrick part. BioBrick Placeholders accomplish this task by providing internal restriction sites compatible with the standard BioBrick restriction sites.

 

BioPlastic

Growing a renewable resource

Polyhydroybutyrate (PHB) is a polykydroxyalkanoate (PHA), a polyester polymer that is naturally synthesized by certain microorganisms. There is enormous potential for PHB production as a plastic material due to the fact that it has similar physical properties of polypropylene, a ubiquitous thermoplastic polymer derived from petroleum. Furthermore, PHB is biologically degradable.

 

Additions to the Registry

Giving back

In addition to adding our Genetic Attenuators, BioBrick Placeholders and bioplastic parts to the Registry, we have also submitted parts that code for orange fluorescent protein (OFP), strongly enhanced blue fluorescent protein (SBFP2) and streptomycin 3'-adenyltransferase, which enables resistance to the antibiotic streptomycin