Team:Princeton/ListofPlasmidDesigns

From 2008.igem.org

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(New page: {{PrincetonHeader}} The highlight code is as follows: style="background:yellow; color:black" | yellow: overlap of primers with the DNA style="background:green; color:black" |dark green:...)
 
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The highlight code is as follows:
The highlight code is as follows:
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  style="background:yellow; color:black" | yellow: overlap of primers with the DNA
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  {|align="left"
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  style="background:green; color:black" |dark green: restriction site
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|style="background:yellow; color:black" | yellow: overlap of primers with the DNA
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  style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand.
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  |}
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  style="background:cyan; color:black" |light blue: kozak sequence.
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  style="background:teal; color:black" |dark blue: stop codon.
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{|align="left"
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  style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell)
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|style="background:green; color:black" |dark green: restriction site
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  style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.
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  |}
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{|align="left"
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|style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand.
 +
  |}
 +
 
 +
{|align="left"
 +
|style="background:cyan; color:black" |light blue: kozak sequence.
 +
  |}
 +
 
 +
{|align="left"
 +
|style="background:teal; color:black" |dark blue: stop codon.
 +
  |}
 +
 
 +
{|align="left"
 +
|style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell)
 +
  |}
 +
 
 +
{|align="left"
 +
|style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.
 +
|}
 +
 
 +
 
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{|align="center"
{|align="center"
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|[[pLV-Hef1aLacO-MashI-2A-ChAT-Ubc-Hyg]]  
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|[https://2008.igem.org/Media:pLV-Hef1aLacO-MashI-2A-ChAT-Ubc-Hyg.pdf pLV-Hef1aLacO-MashI-2A-ChAT-Ubc-Hyg]
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[[pLV-Hef1aLacO-MashI-2A-Nurr1-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1aLacO-MashI-2A-Nurr1-Ubc-Hyg.pdf pLV-Hef1aLacO-MashI-2A-Nurr1-Ubc-Hyg]
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[[pLV-Hef1a-LacO-MashI-Cerulean-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1a-LacO-MashI-Cerulean-Ubc-Hyg.pdf pLV-Hef1a-LacO-MashI-Cerulean-Ubc-Hyg]
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[[pLV-Hef1a-LacO-MashI-EGFP-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1a-LacO-MashI-EGFP-Ubc-Hyg.pdf pLV-Hef1a-LacO-MashI-EGFP-Ubc-Hyg]
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[[pLV-Hef1a-LacO-MashI-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1a-LacO-MashI-Ubc-Hyg.pdf pLV-Hef1a-LacO-MashI-Ubc-Hyg]
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[[pLV-Hef1a-LacO-MashI-Ubc-Hyg2]]  
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[https://2008.igem.org/Media:pLV-Hef1a-LacO-MashI-Ubc-Hyg2.pdf pLV-Hef1a-LacO-MashI-Ubc-Hyg2]
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[[pLV-Hef1aLacO-Ngn1-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1aLacO-Ngn1-Ubc-Hyg.pdf pLV-Hef1aLacO-Ngn1-Ubc-Hyg]
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[[pLV-Hef1aLacO-Ngn2-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1aLacO-Ngn2-Ubc-Hyg.pdf pLV-Hef1aLacO-Ngn2-Ubc-Hyg]
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[[pLV-Hef1aLacO-Nurr1-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1aLacO-Nurr1-Ubc-Hyg.pdf pLV-Hef1aLacO-Nurr1-Ubc-Hyg]
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[[pLV-Hef1aLacO-Tlx3-Ubc-Hyg]]  
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[https://2008.igem.org/Media:pLV-Hef1aLacO-Tlx3-Ubc-Hyg.pdf pLV-Hef1aLacO-Tlx3-Ubc-Hyg]
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[[pLV-pLux-ChAT-Ubc-Neo]]  
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[https://2008.igem.org/Media:pLV-pLux-ChAT-Ubc-Neo.pdf pLV-pLux-ChAT-Ubc-Neo]
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[[pLV-TRE-Cav3.1]]  
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[https://2008.igem.org/Media:pLV-TRE-Cav3.1.pdf pLV-TRE-Cav3.1]
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[[pLV-TRE-Cav3.1-Ubc-Bleo]]  
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[https://2008.igem.org/Media:pLV-TRE-Cav3.1-Ubc-Bleo.pdf pLV-TRE-Cav3.1-Ubc-Bleo]
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[[pLV-TRE-D5R-Ubc-Bleo]]  
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[https://2008.igem.org/Media:pLV-TRE-D5R-Ubc-Bleo.pdf pLV-TRE-D5R-Ubc-Bleo]
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 +
[https://2008.igem.org/Media:pLV-TRE-GLRA1-2A-gephyrin-Ubc-Neo.pdf pLV-TRE-GLRA1-2A-gephyrin-Ubc-Neo]
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[[pLV-TRE-GLRA1-2A-gephyrin-Ubc-Neo]]  
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[https://2008.igem.org/Media:pLV-TRE-GLRA1-Ubc-Neo.pdf pLV-TRE-GLRA1-Ubc-Neo]
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[[pLV-TRE-GLRA1-Ubc-Neo]]  
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[https://2008.igem.org/Media:pLV-TRE-GLYT2-2A-Viaat-Ubc-Bla.pdf pLV-TRE-GLYT2-2A-Viaat-Ubc-Bla]
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[[pLV-TRE-GLYT2-2A-Viaat-Ubc-Bla]]  
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[https://2008.igem.org/Media:pLV-TRE-HCN2-Ubc-Bla.pdf pLV-TRE-HCN2-Ubc-Bla]
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[[pLV-TRE-HCN2-Ubc-Bla]]  
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[https://2008.igem.org/Media:pLV-TRE-Lbx1-2A-GLRA1-2A-D5R-Ubc-Bla.pdf pLV-TRE-Lbx1-2A-GLRA1-2A-D5R-Ubc-Bla]
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[[pLV-TRE-Lbx1-2A-GLRA1-2A-D5R-Ubc-Bla]]  
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[https://2008.igem.org/Media:pLV-TRE-Lbx1-Ubc-Puro.pdf pLV-TRE-Lbx1-Ubc-Puro]
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[[pLV-TRE-Lbx1-Ubc-Puro]]  
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[https://2008.igem.org/Media:pLV-TRE-MashI-IRES2-mKate.pdf pLV-TRE-MashI-IRES2-mKate]
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[[pLV-TRE-MashI-IRES2-mKate]]  
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[https://2008.igem.org/Media:pLV-TRE-Neuregulin1-Ubc-Neo.pdf pLV-TRE-Neuregulin1-Ubc-Neo]
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[[pLV-TRE-Neuregulin1-Ubc-Neo]]  
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[https://2008.igem.org/Media:pLV-TRE-Ngn1-EYFP-Ubc-Bla.pdf pLV-TRE-Ngn1-EYFP-Ubc-Bla]
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[[pLV-TRE-Ngn1-EYFP-Ubc-Bla]]
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[https://2008.igem.org/Media:pLV-TRE-Ngn1-EYFP-2A-mKate-Ubc-Bla.pdf pLV-TRE-Ngn1-EYFP-2A-mKate-Ubc-Bla]
-
[[pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro]]
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[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro]
-
[[pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro]]
+
[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro]
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}
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|}

Latest revision as of 03:58, 30 October 2008

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PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




The highlight code is as follows:

yellow: overlap of primers with the DNA
dark green: restriction site
pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand.
light blue: kozak sequence.
dark blue: stop codon.
gray: a 2A sequence (the amino acid sequence is cut by the cell)
red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.





pLV-Hef1aLacO-MashI-2A-ChAT-Ubc-Hyg

pLV-Hef1aLacO-MashI-2A-Nurr1-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Cerulean-Ubc-Hyg

pLV-Hef1a-LacO-MashI-EGFP-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Ubc-Hyg2

pLV-Hef1aLacO-Ngn1-Ubc-Hyg

pLV-Hef1aLacO-Ngn2-Ubc-Hyg

pLV-Hef1aLacO-Nurr1-Ubc-Hyg

pLV-Hef1aLacO-Tlx3-Ubc-Hyg

pLV-pLux-ChAT-Ubc-Neo

pLV-TRE-Cav3.1

pLV-TRE-Cav3.1-Ubc-Bleo

pLV-TRE-D5R-Ubc-Bleo

pLV-TRE-GLRA1-2A-gephyrin-Ubc-Neo

pLV-TRE-GLRA1-Ubc-Neo

pLV-TRE-GLYT2-2A-Viaat-Ubc-Bla

pLV-TRE-HCN2-Ubc-Bla

pLV-TRE-Lbx1-2A-GLRA1-2A-D5R-Ubc-Bla

pLV-TRE-Lbx1-Ubc-Puro

pLV-TRE-MashI-IRES2-mKate

pLV-TRE-Neuregulin1-Ubc-Neo

pLV-TRE-Ngn1-EYFP-Ubc-Bla

pLV-TRE-Ngn1-EYFP-2A-mKate-Ubc-Bla

pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro

pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro