Team:Chiba/jk

From 2008.igem.org

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>[[Team:Chiba/Internal |Notebook]]  
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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[[Image:Chiba-U.gif|center]]
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
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!align="center"|[[Team:Chiba/j|ホーム]]
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!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
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!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
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!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
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!align="center"|[[Team:Chiba/Internal|ノート]]
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{| align="center"
 
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|{{#calendar: year=2008 | month=08 | title=Team:Chiba/Calendar-Main}}
 
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|{{#calendar: year=2008 | month=09 | title=Team:Chiba/Calendar-Main}}
 
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|{{#calendar: year=2008 | month=10 | title=Team:Chiba/Calendar-Main}}
 
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|{{#calendar: year=2008 | month=11 | title=Team:Chiba/Calendar-Main}}
 
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|
 
|}
|}
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<table border=0 width="100%"><tr><td width="65%" valign="top"><div style="background:#aaaaaa;padding:3px;">
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<!-- 左側コンテンツのタイトル -->
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カレンダー
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</div><div style="background:#dddddd;padding:5px;">
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<!-- 左側コンテンツの内容 -->
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----
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{|  align="center"
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{| style="color:#0c6;" cellpadding="2" cellspacing="1" border="1" bordercolor="#800000" width="60%" align="center"
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!align="center"|{{#calendar: title=Team:Chiba/Calendar-Home |year=2008 | month=08}}
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!align="center"|{{#calendar: title=Team:Chiba/Calendar-Home |year=2008 | month=09}}
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|}
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{| style="color:#0c6;" cellpadding="2" cellspacing="1" border="1" bordercolor="#800000" width="60%" align="center"
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!align="center"|{{#calendar: title=Team:Chiba/Calendar-Home |year=2008 | month=10}}
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!align="center"|{{#calendar: title=Team:Chiba/Calendar-Home |year=2008 | month=11}}
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|}
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==To Do List==
 
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*出力の決定（杉山、福冨）
 
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**LacZ-->基質濃度に依存
 
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**rLuciferine-->見えない？-->基質が必要、光分解性（詳しく調べる）
 
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*Rs,PFL(LuxR,J3720-->imperial,2006)の作成（杉山、福冨）
 
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*入力の決定（香取）
 
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**Arabinose promotor?-->線形成？On/Offはっきり？
 
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**On/Offの必要性は-->なし？
 
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*Time-delayの確認（井山、川崎、小林、冨永）
 
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**cinIのクローニング（井山、川崎）<Br>
 
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**差を広げる-->温度依存性、培地依存性.PFL(Positive feedback loop)(BBa_J37015,BBa_J37020)の利用.
 
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**AHL固定(1nM,10nM,100nM~)-->閾値（10nM付近以上）を超えると、GFPによる蛍光強度は変わらない。4~5時間で飽和する。
 
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**固体培地にspot
 
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**His TagつきGFP(その他タンパク質)を発現-->freeze-->SDS-PAGE-->blotting
 
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**Is(BW,XL10G)をT9002(BW)でdelay check
 
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***さらに-BBa_J37020を形質転換(XL10G,BW)でtime-delayをチェック
 
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*work out deal to print T-shirts (井山、福冨)
 
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===still under discussion===
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</div></td><td width="1%"><!-- spacing --></td><td width="34%" valign="top"><div style="background:#aaaaaa; padding:3px;">
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*出力を交換できる形でクローニングしよう-->intermideatesの作成
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<!-- 右側コンテンツのタイトル -->
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*AHL synthase のkineticsについての情報は？-->[[Team:Chiba/Reference/j|ref.10 and ref.11]]
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スケジュール
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*ベクターの乗せ換え(PSB1-->P15A)（井山、小林、川崎）<Br>
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</div><div style="background:#dddddd;padding:5px;">
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<!-- 右側コンテンツの内容 -->
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__NOTOC__
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===連絡===
 
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実験結果より
 
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-->Rs濃度が律速→PFL(J37020)使う？Rs替える？
 
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----
 
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{|class=wikitable align=center width=900
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=== 実験日程 ===
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*'''17, August~'''
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より詳細な実験ノートは英語版カレンダーから参照できます。
 +
:*[[Team:Chiba/jk|日本語版実験ノート]]
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::[[Team:Chiba/jk/α|入力班]]
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::[[Team:Chiba/jk/β|送受信班]]
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::[[Team:Chiba/jk/γ|出力班]]
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:*[[Team:Chiba/protocol/j|プロトコル]]
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:*[[Team:Chiba/Reference/j|リファレンス]]
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!<big>[[Team:Chiba/jk/α|入力班]]</big>
 
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!<big>[[Team:Chiba/jk/β|送受信班]]</big>
 
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!<big>[[Team:Chiba/jk/γ|出力班]]</big>
 
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!<big>その他</big>
 
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|-
 
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| align=center|
 
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小林あおい Aoi Kobayashi
 
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香取崇広 Takahiro Katori
 
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|align=center|
 
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冨永将大 Masahiro Tominaga
 
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川崎浩平 Kohei Kawasaki
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</div></td></tr></table>
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井山佳美 Yoshimi Iyama
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== 実験班 ==
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*''17, August ~ 13, September''
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{|class=wikitable align=center width=900
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!<big>入力班</big>
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!<big>通信班</big>
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!<big>出力班</big>
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|-
| align=center|
| align=center|
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福冨浩樹 Hiroki Fukutomi
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[[User:Takahiro| 香取崇広]], [[User:Aoi| 小林あおい]]
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| align=center|
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[[User:Kohei| 川崎浩平]], [[User:Yoshimi| 井山佳美]], [[User:Masahiro| 冨永将大]]
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| align=center|
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[[User:Hiroki| 福冨浩樹]], [[User:Mai| 杉山まい]]
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|}
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杉山まい Mai Sugiyama
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*''13, September ~ 1, October''
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{|class=wikitable align=center width=900
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!<big>通信班</big>
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!<big>受信班</big>
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|-
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| align=center|
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[[User:Kohei| 川崎浩平]], [[User:Yoshimi| 井山佳美]], [[User:Takahiro| 香取崇広]], [[User:Masahiro| 冨永将大]]
| align=center|
| align=center|
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*[[Team:Chiba/Reference/j|Reference]]
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[[User:Hiroki| 福冨浩樹]], [[User:Mai| 杉山まい]], [[User:Aoi| 小林あおい]]
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*[[Team:Chiba/protocol|Protocols]]
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*Inventory
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*[[Team:Chiba/Ordering|Ordering]]
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|}
|}
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----
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*''1, October~''
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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{|class=wikitable align=center width=900
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!<big>受信班</big>
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!<big>受信側デモ班</big>
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!<big>送信側デモ班</big>
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|-
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| align=center|
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[[User:Aoi| 小林あおい]], [[User:Hiroki| 福冨浩樹]]
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| align=center|
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[[User:Kohei| 川崎浩平]], [[User:Takahiro| 香取崇広]]
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| align=center|
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[[User:Yoshimi| 井山佳美]] 
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|}
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
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!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
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|}
|}

Latest revision as of 08:14, 14 December 2008

Chiba-U.gif
ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート

カレンダー

August
MTWTFSS
        [http://2008.igem.org/Team:Chiba/Calendar-Home/1_August_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Home/2_August_2008 2] [http://2008.igem.org/Team:Chiba/Calendar-Home/3_August_2008 3]
[http://2008.igem.org/Team:Chiba/Calendar-Home/4_August_2008 4] [http://2008.igem.org/Team:Chiba/Calendar-Home/5_August_2008 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/6_August_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/7_August_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/8_August_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/10_August_2008&action=edit 10]
[http://2008.igem.org/Team:Chiba/Calendar-Home/11_August_2008 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/12_August_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/13_August_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/14_August_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/15_August_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/16_August_2008&action=edit 16] [http://2008.igem.org/Team:Chiba/Calendar-Home/17_August_2008 17]
[http://2008.igem.org/Team:Chiba/Calendar-Home/18_August_2008 18] [http://2008.igem.org/Team:Chiba/Calendar-Home/19_August_2008 19] [http://2008.igem.org/Team:Chiba/Calendar-Home/20_August_2008 20] [http://2008.igem.org/Team:Chiba/Calendar-Home/21_August_2008 21] [http://2008.igem.org/Team:Chiba/Calendar-Home/22_August_2008 22] [http://2008.igem.org/Team:Chiba/Calendar-Home/23_August_2008 23] [http://2008.igem.org/Team:Chiba/Calendar-Home/24_August_2008 24]
[http://2008.igem.org/Team:Chiba/Calendar-Home/25_August_2008 25] [http://2008.igem.org/Team:Chiba/Calendar-Home/26_August_2008 26] [http://2008.igem.org/Team:Chiba/Calendar-Home/27_August_2008 27] [http://2008.igem.org/Team:Chiba/Calendar-Home/28_August_2008 28] [http://2008.igem.org/Team:Chiba/Calendar-Home/29_August_2008 29] [http://2008.igem.org/Team:Chiba/Calendar-Home/30_August_2008 30] [http://2008.igem.org/Team:Chiba/Calendar-Home/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Team:Chiba/Calendar-Home/1_September_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Home/2_September_2008 2] [http://2008.igem.org/Team:Chiba/Calendar-Home/3_September_2008 3] [http://2008.igem.org/Team:Chiba/Calendar-Home/4_September_2008 4] [http://2008.igem.org/Team:Chiba/Calendar-Home/5_September_2008 5] [http://2008.igem.org/Team:Chiba/Calendar-Home/6_September_2008 6] [http://2008.igem.org/Team:Chiba/Calendar-Home/7_September_2008 7]
[http://2008.igem.org/Team:Chiba/Calendar-Home/8_September_2008 8] [http://2008.igem.org/Team:Chiba/Calendar-Home/9_September_2008 9] [http://2008.igem.org/Team:Chiba/Calendar-Home/10_September_2008 10] [http://2008.igem.org/Team:Chiba/Calendar-Home/11_September_2008 11] [http://2008.igem.org/Team:Chiba/Calendar-Home/12_September_2008 12] [http://2008.igem.org/Team:Chiba/Calendar-Home/13_September_2008 13] [http://2008.igem.org/Team:Chiba/Calendar-Home/14_September_2008 14]
[http://2008.igem.org/Team:Chiba/Calendar-Home/15_September_2008 15] [http://2008.igem.org/Team:Chiba/Calendar-Home/16_September_2008 16] [http://2008.igem.org/Team:Chiba/Calendar-Home/17_September_2008 17] [http://2008.igem.org/Team:Chiba/Calendar-Home/18_September_2008 18] [http://2008.igem.org/Team:Chiba/Calendar-Home/19_September_2008 19] [http://2008.igem.org/Team:Chiba/Calendar-Home/20_September_2008 20] [http://2008.igem.org/Team:Chiba/Calendar-Home/21_September_2008 21]
[http://2008.igem.org/Team:Chiba/Calendar-Home/22_September_2008 22] [http://2008.igem.org/Team:Chiba/Calendar-Home/23_September_2008 23] [http://2008.igem.org/Team:Chiba/Calendar-Home/24_September_2008 24] [http://2008.igem.org/Team:Chiba/Calendar-Home/25_September_2008 25] [http://2008.igem.org/Team:Chiba/Calendar-Home/26_September_2008 26] [http://2008.igem.org/Team:Chiba/Calendar-Home/27_September_2008 27] [http://2008.igem.org/Team:Chiba/Calendar-Home/28_September_2008 28]
[http://2008.igem.org/Team:Chiba/Calendar-Home/29_September_2008 29] [http://2008.igem.org/Team:Chiba/Calendar-Home/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Team:Chiba/Calendar-Home/1_October_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Home/2_October_2008 2] [http://2008.igem.org/Team:Chiba/Calendar-Home/3_October_2008 3] [http://2008.igem.org/Team:Chiba/Calendar-Home/4_October_2008 4] [http://2008.igem.org/Team:Chiba/Calendar-Home/5_October_2008 5]
[http://2008.igem.org/Team:Chiba/Calendar-Home/6_October_2008 6] [http://2008.igem.org/Team:Chiba/Calendar-Home/7_October_2008 7] [http://2008.igem.org/Team:Chiba/Calendar-Home/8_October_2008 8] [http://2008.igem.org/Team:Chiba/Calendar-Home/9_October_2008 9] [http://2008.igem.org/Team:Chiba/Calendar-Home/10_October_2008 10] [http://2008.igem.org/Team:Chiba/Calendar-Home/11_October_2008 11] [http://2008.igem.org/Team:Chiba/Calendar-Home/12_October_2008 12]
[http://2008.igem.org/Team:Chiba/Calendar-Home/13_October_2008 13] [http://2008.igem.org/Team:Chiba/Calendar-Home/14_October_2008 14] [http://2008.igem.org/Team:Chiba/Calendar-Home/15_October_2008 15] [http://2008.igem.org/Team:Chiba/Calendar-Home/16_October_2008 16] [http://2008.igem.org/Team:Chiba/Calendar-Home/17_October_2008 17] [http://2008.igem.org/Team:Chiba/Calendar-Home/18_October_2008 18] [http://2008.igem.org/Team:Chiba/Calendar-Home/19_October_2008 19]
[http://2008.igem.org/Team:Chiba/Calendar-Home/20_October_2008 20] [http://2008.igem.org/Team:Chiba/Calendar-Home/21_October_2008 21] [http://2008.igem.org/Team:Chiba/Calendar-Home/22_October_2008 22] [http://2008.igem.org/Team:Chiba/Calendar-Home/23_October_2008 23] [http://2008.igem.org/Team:Chiba/Calendar-Home/24_October_2008 24] [http://2008.igem.org/Team:Chiba/Calendar-Home/25_October_2008 25] [http://2008.igem.org/Team:Chiba/Calendar-Home/26_October_2008 26]
[http://2008.igem.org/Team:Chiba/Calendar-Home/27_October_2008 27] [http://2008.igem.org/Team:Chiba/Calendar-Home/28_October_2008 28] [http://2008.igem.org/Team:Chiba/Calendar-Home/29_October_2008 29] [http://2008.igem.org/Team:Chiba/Calendar-Home/30_October_2008 30] [http://2008.igem.org/Team:Chiba/Calendar-Home/31_October_2008 31]
November
MTWTFSS
          [http://2008.igem.org/Team:Chiba/Calendar-Home/1_November_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Home/2_November_2008 2]
[http://2008.igem.org/Team:Chiba/Calendar-Home/3_November_2008 3] [http://2008.igem.org/Team:Chiba/Calendar-Home/4_November_2008 4] [http://2008.igem.org/Team:Chiba/Calendar-Home/5_November_2008 5] [http://2008.igem.org/Team:Chiba/Calendar-Home/6_November_2008 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/7_November_2008&action=edit 7] [http://2008.igem.org/Team:Chiba/Calendar-Home/8_November_2008 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/9_November_2008&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/10_November_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/11_November_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/12_November_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/13_November_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/14_November_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/15_November_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/16_November_2008&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/17_November_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/18_November_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/19_November_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/20_November_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/21_November_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/22_November_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/23_November_2008&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/24_November_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/25_November_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/26_November_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/27_November_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/28_November_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/29_November_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Home/30_November_2008&action=edit 30]


スケジュール


実験日程

  • 17, August~

より詳細な実験ノートは英語版カレンダーから参照できます。

  • 日本語版実験ノート
入力班
送受信班
出力班



実験班

  • 17, August ~ 13, September
入力班 通信班 出力班

香取崇広, 小林あおい

川崎浩平, 井山佳美, 冨永将大

福冨浩樹, 杉山まい

  • 13, September ~ 1, October
通信班 受信班

川崎浩平, 井山佳美, 香取崇広, 冨永将大

福冨浩樹, 杉山まい, 小林あおい

  • 1, October~
受信班 受信側デモ班 送信側デモ班

小林あおい, 福冨浩樹

川崎浩平, 香取崇広

井山佳美


ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk"