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- | '''17,August 2008'''
| + | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> |
- | <br>PCR | + | |
- | <br>BBa_E0430① | + | |
- | <br>BBa_E0420②
| + | |
- | <br>BBa_J06702③
| + | |
- | <br>BBa_S01416④
| + | |
- | <br>BBa_I73003⑤
| + | |
- | <br>BBa_I73004⑥
| + | |
- | <br>BBa_I730026⑦
| + | |
- | <br>BBa_I763002⑧
| + | |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>FW primer</td>
| + | |
- | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>VR primer</td>
| + | |
- | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dNTPmix</td>
| + | |
- | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>thermo pol buffer</td>
| + | |
- | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA pol(VENT)</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dH2O</td>
| + | |
- | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
| + | |
- | </tr>
| + | |
- | </table> | + | |
| | | |
- | '''20,August 2008'''
| + | >[[Team:Chiba/Internal|ノートに戻る]] |
- | <br>TRANSFORMATION
| + | |
- | <br>BBa_J52008(Luciferase Renilla)-->colony
| + | |
- | <br>BBa_I712019(Luciferase Fire fly)-->no colony
| + | |
- | <br>BBa_J332002(LacZ)-->colony
| + | |
- | <br>BBa_E2030(Venus YFP)-->not so good
| + | |
- | <br>YFP(P-GEX)-->colony
| + | |
- | <br>BBa_F2620-->colony
| + | |
- | <br>BBa_T9002-->colony
| + | |
| | | |
| | | |
- | '''21,August 2008'''
| + | == Out put team == |
- | <br>TRANSFORMATION
| + | |
- | <br>BBa_J32007(Lux CDABE)-->no colony
| + | |
- | <br>BBa_B0034(RBS)-->colony
| + | |
| | | |
- | '''22,August 2008'''
| |
- | <br>MINI PREP
| |
- | <br>BBa_J52008(Luciferase)
| |
- | <br>BBa_F2620
| |
- | <br>BBa_T9002
| |
- | <br>BBa_J33202(LacZ)
| |
- | <br>PUC19
| |
- | <br>PGEX VENUS YFP(GST fusion)
| |
- | <br>
| |
- | <br>TRANSFORMATION
| |
- | <br>PGEX VENUS YFP(GST fusion)
| |
| | | |
- | '''24,August 2008'''
| + | ===実験ノート=== |
- | <br>TRANSFORMATION
| + | |
- | <br>BBa_J63001(yeast optimized YFP)-->colony
| + | |
- | <br>Digestion
| + | |
- | <br>BBa_F2620(1)①
| + | |
- | <br>BBa_F2620(2)②
| + | |
- | <br>Menbren Venus YFP③
| + | |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①</td><td>②</td><td>③</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>3</td><td>3</td><td>3</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>Buffer3</td>
| + | |
- | <td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dH2O</td>
| + | |
- | <td>6</td><td>6</td><td>6</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>EcoRⅠ</td>
| + | |
- | <td>0.5</td><td>0.5</td><td>0.5</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>->37℃ 30min
| + | |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>loading Dye</td>
| + | |
- | <td>2</td><td>2</td><td>2</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>12</td><td>12</td><td>12</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①</td><td>②</td><td>③</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>2</td><td>2</td><td>2</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>loading Dye</td>
| + | |
- | <td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dH2O</td>
| + | |
- | <td>3</td><td>3</td><td>3</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>6</td><td>6</td><td>6</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>->135V 30min
| + | |
| | | |
- | '''25,August,2008'''
| + | *[[Team:Chiba/jk/γ/week 1|week 1 (17,August~23,August)]] |
- | <br>PCR
| + | *[[Team:Chiba/jk/γ/week 2|week 2 (24,August~30,August)]] |
- | <br>BBa_E2030①
| + | *[[Team:Chiba/jk/γ/week 3|week 3 (31,August~6,September)]] |
- | <br>BBa_J33202②
| + | *[[Team:Chiba/jk/γ/week 4|week 4 (7,September~13,September)]] |
- | <br>BL21③
| + | *[[Team:Chiba/jk/γ/week 5|week 5 (14,September~20,September)]] |
- | <br>BBa_J52008④
| + | *[[Team:Chiba/jk/γ/week 6|week 6 (21,September~27,September)]] |
- | <br>BBa_I712019⑤
| + | *[[Team:Chiba/jk/γ/week 7|week 7 (28,September~4,October)]] |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | *[[Team:Chiba/jk/γ/week 7|week 7 (5,October~11,October)]] |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>FW primer</td>
| + | |
- | <td>5(Venus_YFP_fwd)</td><td>5(LacZ_fwd)</td><td>5(LacZ_fwd)</td><td>5(rLUC_fwd)</td><td>5(fLuc_fwd)</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>VR primer</td>
| + | |
- | <td>5(VR)</td><td>5(LacZ_rev)</td><td>5(LacZ_rev)</td><td>5(VR)</td><td>5(VR)</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dNTPmix</td>
| + | |
- | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>thermo pol buffer</td>
| + | |
- | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA pol(VENT)</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dH2O</td>
| + | |
- | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>->Gel
| + | |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| + | |
- | <tr>
| + | |
- | <td width="257">Sample No</td>
| + | |
- | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>DNA tamplate</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>loading Dye</td>
| + | |
- | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>dH2O</td>
| + | |
- | <td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>TOTAL</td>
| + | |
- | <td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
| | | |
- | '''25,August,2008'''
| |
- | <br>MINI prep
| |
- | <br>Venus
| |
- | <br>BBa_F2620
| |
- | <br>TRANSFORMATRION
| |
- | <br>Venus
| |
- | <br>PUC19
| |
- | <br>pGFPUV
| |
- | <br>pGEX Venus YFP
| |
- | <br>Membren Venus YFP
| |
- | <br>PCR
| |
- | <br>BBa_J63001①
| |
- | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
- | <tr>
| |
- | <td width="257">Sample No</td>
| |
- | <td>①</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>DNA tamplate</td>
| |
- | <td>1</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>FW primer(Venus)</td>
| |
- | <td>5</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>VR primer</td>
| |
- | <td>5</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>dNTPmix</td>
| |
- | <td>10</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>thermo pol buffer</td>
| |
- | <td>10</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>DNA pol(VENT)</td>
| |
- | <td>1</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>dH2O</td>
| |
- | <td>68</td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>TOTAL</td>
| |
- | <td>100</td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>Digestion
| |
| | | |
| | | |
| + | ===Time Responce Test=== |
| | | |
| + | <br>>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]] |
| + | <br>>[[Team:Chiba/jk/γ/trs|Time Responce Solid]] |
| | | |
- | Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620
| |
- | :結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
| |
- | ::失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
| |
- | *'''2008,8,21(thu)'''
| |
- | Lux CDABE(J32007:2007),RBS(B0034:2007)
| |
- | :結果:成功→RBS
| |
- | ::失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功
| |
- | *'''2008,8,22(sun)'''
| |
- | Venus YFP
| |
- | :結果
| |
- | *'''2008,8,22(fri)'''
| |
- | J52008(2007),F2620,T9002,PUC19(LacZ),VenusYFP,J33202(2007)
| |
- | :結果:ゲル電→J33202はバンドの位置が違っていましたが、大丈夫だと思われます。他は正しいバンドがでました。
| |
| | | |
| | | |
| | | |
- | | + | {| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" | |
- | {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center" | + | |
| !align="center"|[[Team:Chiba/j|ホーム]] | | !align="center"|[[Team:Chiba/j|ホーム]] |
| !align="center"|[[Team:Chiba/Team/j|メンバー紹介]] | | !align="center"|[[Team:Chiba/Team/j|メンバー紹介]] |
| !align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]] | | !align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]] |
- | !align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]] | + | !align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]] |
- | !align="center"|[[Team:Chiba/Modeling/j|モデリング]]
| + | |
| !align="center"|[[Team:Chiba/Internal|ノート]] | | !align="center"|[[Team:Chiba/Internal|ノート]] |
| |} | | |} |