Team:Chiba/jk/γ

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>[[Team:Chiba/jk|実験ログ]]<Br>
+
<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
-
*[[Team:Chiba/jk/β/weak 1|week 1]]
+
>[[Team:Chiba/Internal|ノートに戻る]]
-
*[[Team:Chiba/jk/β/weak 2|week 2]]
+
-
*[[Team:Chiba/jk/β/weak 3|week 3]]
+
-
*[[Team:Chiba/jk/β/weak 4|week 4]]
+
-
*[[Team:Chiba/jk/β/weak 5|week 5]]
+
-
*[[Team:Chiba/jk/β/weak 6|week 6]]
+
-
----
 
-
'''17,August 2008'''
 
-
<br>PCR
 
-
<br>BBa_E0430①
 
-
<br>BBa_E0420②
 
-
<br>BBa_J06702③
 
-
<br>BBa_S01416④
 
-
<br>BBa_I73003⑤
 
-
<br>BBa_I73004⑥
 
-
<br>BBa_I730026⑦
 
-
<br>BBa_I763002⑧
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>FW primer</td>
 
-
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>VR primer</td>
 
-
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dNTPmix</td>
 
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>thermo pol buffer</td>
 
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA pol(VENT)</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
 
-
</tr>
 
-
</table>
 
 +
== Out put team ==
-
----
 
-
'''20,August 2008'''
 
-
<br>TRANSFORMATION
 
-
<br>BBa_J52008(Luciferase Renilla)-->colony
 
-
<br>BBa_I712019(Luciferase Fire fly)-->no colony
 
-
<br>BBa_J332002(LacZ)-->colony
 
-
<br>BBa_E2030(Venus YFP)-->not so good
 
-
<br>YFP(P-GEX)-->colony
 
-
<br>BBa_F2620-->colony
 
-
<br>BBa_T9002-->colony
 
 +
===実験ノート===
-
----
+
*[[Team:Chiba/jk/γ/week 1|week 1  (17,August~23,August)]]
-
'''21,August 2008'''
+
*[[Team:Chiba/jk/γ/week 2|week 2  (24,August~30,August)]]
-
<br>TRANSFORMATION
+
*[[Team:Chiba/jk/γ/week 3|week 3  (31,August~6,September)]]
-
<br>BBa_J32007(Lux CDABE)-->no colony
+
*[[Team:Chiba/jk/γ/week 4|week 4  (7,September~13,September)]]
-
<br>BBa_B0034(RBS)-->colony
+
*[[Team:Chiba/jk/γ/week 5|week 5  (14,September~20,September)]]
 +
*[[Team:Chiba/jk/γ/week 6|week 6  (21,September~27,September)]]
 +
*[[Team:Chiba/jk/γ/week 7|week 7  (28,September~4,October)]]
 +
*[[Team:Chiba/jk/γ/week 7|week 7  (5,October~11,October)]]
-
----
 
-
'''22,August 2008'''
 
-
<br>MINI PREP
 
-
<br>BBa_J52008(Luciferase)
 
-
<br>BBa_F2620
 
-
<br>BBa_T9002
 
-
<br>BBa_J33202(LacZ)
 
-
<br>PUC19
 
-
<br>PGEX VENUS YFP(GST fusion)
 
-
<br>
 
-
<br>TRANSFORMATION
 
-
<br>PGEX VENUS YFP(GST fusion)
 
-
----
+
===Time Responce Test===
-
'''24,August 2008'''
+
-
<br>TRANSFORMATION
+
-
<br>BBa_J63001(yeast optimized YFP)-->colony
+
-
<br>Digestion
+
-
<br>BBa_F2620(1)①
+
-
<br>BBa_F2620(2)②
+
-
<br>Menbren Venus YFP③
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>3</td><td>3</td><td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3</td>
+
-
<td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>0.5</td><td>0.5</td><td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃ 30min
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading Dye</td>
+
-
<td>2</td><td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td><td>12</td><td>12</td>
+
-
</tr>
+
-
</table>
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>2</td><td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading Dye</td>
+
-
<td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>3</td><td>3</td><td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
</table>
+
-
<br>->135V 30min
+
-
----
+
<br>>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
-
'''25,August,2008'''
+
<br>>[[Team:Chiba/jk/γ/trs|Time Responce Solid]]
-
<br>PCR
+
-
<br>BBa_E2030①
+
-
<br>BBa_J33202②
+
-
<br>BL21③
+
-
<br>BBa_J52008④
+
-
<br>BBa_I712019⑤
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>FW primer</td>
+
-
<td>5(Venus_YFP_fwd)</td><td>5(LacZ_fwd)</td><td>5(LacZ_fwd)</td><td>5(rLUC_fwd)</td><td>5(fLuc_fwd)</td>
+
-
</tr>
+
-
<tr>
+
-
<td>VR primer</td>
+
-
<td>5(VR)</td><td>5(LacZ_rev)</td><td>5(LacZ_rev)</td><td>5(VR)</td><td>5(VR)</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTPmix</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>thermo pol buffer</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA pol(VENT)</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
+
-
</tr>
+
-
</table>
+
-
<br>->Gel
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading Dye</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
</table>
+
-
<br>MINI prep
 
-
<br>Venus
 
-
<br>BBa_F2620
 
-
<br>TRANSFORMATRION
 
-
<br>Venus
 
-
<br>PUC19
 
-
<br>pGFPUV
 
-
<br>pGEX Venus YFP
 
-
<br>Membren Venus YFP
 
-
<br>PCR
 
-
<br>BBa_J63001①
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>FW primer(Venus)</td>
 
-
<td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>VR primer</td>
 
-
<td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dNTPmix</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>thermo pol buffer</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA pol(VENT)</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>68</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>100</td>
 
-
</tr>
 
-
</table>
 
-
----
 
-
'''26,August,2008'''
 
-
<br>Gel check
 
-
<br>BBa_F2620
 
-
<br>BBa_F2620
 
-
<br>BBa_J63001
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
 
-
</tr>
 
-
</table>
 
-
<br>Digestion
 
-
<br>vector:BBa_F2620
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>BBa_F2620</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>100</td>
 
-
</tr>
 
-
<tr>
 
-
<td>SpeⅠ</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA(x10)</td>
 
-
<td>13</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer2</td>
 
-
<td>13</td>
 
-
</tr>
 
-
<td>TOTAL</td>
 
-
<td>130</td>
 
-
</tr>
 
-
</table>
 
-
<br>insert:BBa_J52008,BBa_E2030
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>J52008</td><td>E2030</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>30</td><td>30</td>
 
-
</tr>
 
-
<tr>
 
-
<td>XbaⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DpnⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer3</td>
 
-
<td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA(x10)</td>
 
-
<td>4</td><td>4</td>
 
-
</tr>
 
-
<td>TOTAL</td>
 
-
<td>40</td><td>40</td>
 
-
</tr>
 
-
</table>
 
-
<br>->37℃　3hour
 
-
<br>->SAP
 
-
<br>->37℃ 30min
 
-
<br>->65℃ 15min
 
-
<br>->Zymo Clean
 
-
<br>Ligation
 
-
<br>vector:BBa_F2620 insert:BBa_J52008①
 
-
<br>vector:BBa_F2620 insert:BBa_E2030②
 
-
<br>Negative control:BBa_F2620③
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td>
 
-
</tr>
 
-
<tr>
 
-
<td>vector</td>
 
-
<td>5</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>inser</td>
 
-
<td>10</td><td>7.5</td><td>0</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Ligase Buffer</td>
 
-
<td>4</td><td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Ligase</td>
 
-
<td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>0</td><td>2.5</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>20</td><td>20</td><td>20</td>
 
-
</tr>
 
-
</table>
 
-
<br>->RT 2hour
 
-
<br>->TRANSFORMATION XL10G
 
-
<br>->37℃ over night
 
-
<br>->colony PCR
 
-
<br>->MINI PREP
 
-
----
+
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
-
'''28,August,2008'''
+
-
<br>TIME RESPONCE(liquid)
+
-
 
+
-
----
+
-
'''29,August,2008'''
+
-
<br>TIME RESPONCE(solid)
+
-
 
+
-
----
+
-
'''30,August,2008'''
+
-
<br>TRANSFORMATION
+
-
<br>mcherry
+
-
<br>PCR
+
-
<br>BBa_J52008(rLuc)
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>BBa_J52008</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>rLuc_fwd</td>
+
-
<td>2.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>VR</td>
+
-
<td>2.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>thermo pol Buffer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Vent pol</td>
+
-
<td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>34</td>
+
-
</tr>
+
-
<td>TOTAL</td>
+
-
<td>50</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''31,August,2008'''
+
-
<br>digestion
+
-
<br>BBa_R0010①
+
-
<br>PCR product(BBa_J52008)②
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA </td>
+
-
<td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>SpeⅠ</td>
+
-
<td>1</td><td>0</td>
+
-
</tr>
+
-
<tr>
+
-
<td>PstⅠ</td>
+
-
<td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>XbaⅠ</td>
+
-
<td>0</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA(x100)</td>
+
-
<td>0.5</td><td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer2</td>
+
-
<td>5</td><td>0</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3</td>
+
-
<td>0</td><td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH20</td>
+
-
<td>28.5</td><td>28.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>50</td><td>50</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃ 3hour
+
-
<br>->①20μl refine
+
-
<br>->②20μl refine
+
-
 
+
-
----
+
-
'''1,September,2008'''
+
-
<br>Ligation
+
-
<br>vector:BBa_R0010 insert:BBa_J52008①
+
-
<br>negative control:BBa_R0010②
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td>
+
-
</tr>
+
-
<tr>
+
-
<td>vector</td>
+
-
<td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>insert</td>
+
-
<td>3</td><td>0</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase Buffer</td>
+
-
<td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase</td>
+
-
<td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>3</td><td>5</td>
+
-
</tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td><td>10</td>
+
-
</tr>
+
-
</table>
+
-
<br>->R/T 2hour
+
-
 
+
-
<br>TRANSFORMATION
+
-
<br>BBa_R0079(Las promoter)
+
-
<br>BBa_R0071(RhlR promoter)
+
-
<br>BBa_R0077(cinR promoter+RBS)
+
-
<br>BBa_R0078(cnR promoter)
+
-
<br>BBa_R0062(LUX promoter)
+
-
 
+
-
 
+
-
----
+
-
'''2,September,2008'''
+
-
<br>TIME RESPONCE (Solid)
+
-
 
+
-
<br>COLONY PCR
+
-
<br>BBa_R0010+BBa_J52008
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td>
+
-
</tr>
+
-
<tr>
+
-
<td>culture</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<td>Fwd primer</td>
+
-
<td>1.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Rev primer</td>
+
-
<td>1.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermo pol Buffer</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP mix</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Taq DNA pol (NEB)</td>
+
-
<td>0.2 (1 unit)</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>19.8</td>
+
-
</tr>
+
-
<td>TOTAL</td>
+
-
<td>30</td>
+
-
</tr>
+
-
</table>
+
-
<br>MINI Prep
+
-
<br>BBa_R0079
+
-
<br>BBa_R0071
+
-
<br>BBa_R0077
+
-
<br>BBa_R0078
+
-
<br>BBa_R0062
+
-
 
+
-
----
+
-
'''3,September,2008'''
+
-
<br>Digestion test
+
-
<br>BBa_R0079①
+
-
<br>BBa_R0071②
+
-
<br>BBa_R0077③
+
-
<br>BBa_R0078④
+
-
<br>BBa_R0062⑤
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①~⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>0.1</td>
+
-
</tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃30min
+
-
<br>->Gel cheack
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①~⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''4,Septempber,2008'''
+
-
 
+
-
 
+
-
 
+
-
----
+
-
'''5,September,2008'''
+
-
<br>TIME RESPONCE(liquid)
+
-
<br>Digestion test
+
-
<br>BBa_J52008+BBa_F2620
+
-
<br>BBa_E2030+BBa_F2620
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<br>Luciferase cheack->no light
+
-
 
+
-
----
+
-
'''7,September,2008'''
+
-
<br>Cloning
+
-
<br>Digestion
+
-
<br>vector:BBa_R0040(ptet)① 75ng/μl
+
-
<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
+
-
<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+
-
<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+
-
<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+
-
<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>50</td><td>54</td><td>54</td><td>45</td><td>14</td><td>54</td>
+
-
</tr>
+
-
<tr>
+
-
<td>SpeⅠ</td>
+
-
<td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td>
+
-
</tr>
+
-
<tr>
+
-
<td>PstⅠ</td>
+
-
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>XbaⅠ</td>
+
-
<td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA(x10)</td>
+
-
<td>0.6</td><td>7</td><td>7</td><td>6</td><td>2</td><td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer2</td>
+
-
<td>6</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3</td>
+
-
<td>-</td><td>7</td><td>7</td><td>6</td><td>-</td><td>7<td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>60</td><td>70</td><td>70</td><td>60</td><td>20</td><td>70</td>
+
-
</tr>
+
-
</table>
+
-
<br>----->37℃ 3hour
+
-
<br>----->Gel Extraction
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td><td>2</td><td>2</td><td>2</td><td>1</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td><td>12</td><td>12</td><td>12</td><td>6</td><td>12</td>
+
-
</tr>
+
-
<tr>
+
-
<td>well</td>
+
-
<td>10</td><td>7</td><td>7</td><td>6</td><td>5</td><td>7</td>
+
-
</table>
+
-
<br>->DNA purification->①-④,⑥->7μl ⑤->6μl
+
-
 
+
-
----
+
-
'''8,Septmeber,2008'''
+
-
<br>SAP
+
-
<br>BBa_R0040(ptet)①
+
-
<br>BBa_R0010(pLac)⑤
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>7</td><td>6</td>
+
-
</tr>
+
-
<tr>
+
-
<td>SAP/td>
+
-
<td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>SAP Buffer</td>
+
-
<td>3</td><td>3</td><td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>30</td><td>30</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>30</td><td>30</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃ 1hour
+
-
<br>->65℃ 30min
+
-
<br>->DNA purification
+
-
<br>->21μl  6μl
+
-
<br>->Gel cheack
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye </td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td>
+
-
</tr>
+
-
</table>
+
-
<br>vector:BBa_R0040(ptet)① 75ng/μl
+
-
<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
+
-
<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+
-
<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+
-
<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+
-
<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(vector)</td>
+
-
<td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(insert)</td>
+
-
<td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase Buffer(x5)</td>
+
-
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
+
-
</tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''9,September,2008'''
+
-
 
+
-
----
+
-
'''10,September,2008'''
+
-
<br>TIME Responce(liquid)
+
-
<br>TIME Responce(solid)
+
-
<br>Colony PCR
+
-
<br>->Gel cheack
+
-
<br>MINI Prep
+
-
 
+
-
----
+
-
'''13,September,2008'''
+
-
<br>
+
-
 
+
-
 
+
-
----
+
-
'''14,September,2008'''
+
-
<br>Digestion test
+
-
<br>BBa_Q04510
+
-
<br>BBa_I716463
+
-
<br>BBa_C0051
+
-
<br>BBa_C0070
+
-
<br>BBa_J06620
+
-
<br>BBa_J22141
+
-
<br>BBa_C0076
+
-
<br>BBa_I716542
+
-
<br>BBa_I716462
+
-
<br>BBa_C0078
+
-
<br>BBa_J01101
+
-
<br>BBa_C0071
+
-
<br>BBa_I712019
+
-
<br>BBa_S01416
+
-
<br>BBa_C0079
+
-
<br>BBa_C0077
+
-
<br>BBa_J23007
+
-
<br>BBa_I7100(x2)
+
-
<br>BBa_S01416
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer2(x10)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>6</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>0.075</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃ 1hour
+
-
<br>Gel cheack
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(digestion sample)</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''16,September,2008'''
+
-
<br>Digestion test
+
-
<br>BBa_R0040(ptet)+BBa_S03158(RBS+RhlR)(x2)
+
-
<br>BBa_R0040(ptet)+BBa_S03156(RBS+lasR)(x5)
+
-
<br>BBa_R0040(ptet)+BBa_S03160(RBS+cinR)(x2)
+
-
<br>BBa_S03158(RBS+RhlR)
+
-
<br>BBa_S03156(RBS+lasR)
+
-
<br>BBa_S03160(RBS+cinR)
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
<tr>
+
-
<td>DNA</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>0.083</td>
+
-
</tr>
+
-
<tr>
+
-
<td>PstⅠ</td>
+
-
<td>0.083</td>
+
-
</tr>
+
-
<td>BSA(x10)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3(x10)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td>
+
-
</tr>
+
-
</table>
+
-
<br>->37℃ 1hour
+
-
<br>Gel cheack
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12</td>
+
-
</tr>
+
-
</table>
+
-
<br>TRANSFORATION
+
-
<br>BBa_J37019(LuxI+RBS+LuxR)->no colony
+
-
<br>BBa_J37003(RBS+LuxR)
+
-
<br>BBa_J37020(LuxI+LuxR+pLux+GFP)
+
-
<br>BBa_J37032(pLux+GFP)
+
-
<br>PCR
+
-
<br>Venus YFP(ptet+LuxR+YFP)
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>YFP</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Venus Fwd</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Rev (VR)</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermo pol Buffer</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Vent</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>68</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>100</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''17,September,2008'''
+
-
<br>TRANSFORMATION
+
-
<br>BBa_I13500(RBS+GFP)
+
-
<br>BBa_J37019(Luxpr+LuxR)-->no colony
+
-
<br>MINI Prep
+
-
<br>BBa_J37033(RBS+LuxR)
+
-
<br>BBa_J37020(Luxpr+LuxR+LuxR+GFP)
+
-
<br>BBa_J37032(pLux+GFP)
+
-
<br>Digestion
+
-
<br>BBa_I7100(ptetR+RBS)
+
-
<br>BBa_S03119(ptet+LuxR)
+
-
<br>Ligation product(ptet+LasR)
+
-
<br>Ligation product(ptet+RhlR)
+
-
<br>Ligation product(ptet+cinR)
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA template</td>
+
-
<td>25</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>PstⅠ</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA(x10)</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ Buffer</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>40</td>
+
-
</tr>
+
-
</table>
+
-
<br>-->37℃ 1hour
+
-
<br>->Next day
+
-
----
+
-
'''18,September,2008'''
+
-
<br>SAP 1μl
+
-
<br>->37℃　1hour
+
-
<br>->30min　65℃
+
-
<br>Zymo ->25μl
+
-
<br>Ligation
+
-
<br>vector:BBa_I7100
+
-
<br>insert:BBa_S03119(ptet+LuxR),Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>Vector&insert</td><td>Vector</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(vector)</td>
+
-
<td>3.5</td><td>3.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA(insert)</td>
+
-
<td>7.5</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase</td>
+
-
<td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Ligase Buffer</td>
+
-
<td>4</td><td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td><td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>20</td><td>20</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<br>TRANSFORMATION
+
-
<br>BBa_J54103(RBS+GFP)
+
-
<br>BBa_P2006
+
-
 
+
-
<br>Gel Cheack
+
-
<br>Ligation product(Venus YFP)
+
-
<br>BBa_J37033
+
-
<br>BBa_J37020
+
-
<br>BBa_J37032
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA</td>
+
-
<td>each</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
----
+
-
'''19,September,2008'''
+
-
<br>colony PCR
+
-
<br>ptet+LuxR(x16)
+
-
<br>ptet+LasR(x7)
+
-
<br>ptet+RhlR(x5)
+
-
<br>ptet+cinR(x5)
+
-
 
+
-
 
+
-
----
+
-
'''22,September,2008'''
+
-
<br>Time delay
+
-
 
+
-
----
+
-
'''23,September,2008'''
+
-
<br>Digestion test
+
-
<br>BBa_E0240
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>E0240</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ</td>
+
-
<td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>PstⅠ</td>
+
-
<td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA(x10)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>EcoRⅠ Buffer(x10)</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>10</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<br>Digestion
+
-
<br>Vector:BBa_E0240
+
-
<br>Insert:BBa_R0062
+
-
<br>Vector:BBa_I7100
+
-
<br>Insert:BBa_R0062
+
-
<br>Insert:BBa_J37033
+
-
 
+
-
 
+
-
 
+
-
 
+
-
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
-
!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
+
!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
-
!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
+
!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
|}
|}

Latest revision as of 11:28, 14 December 2008

>ノートに戻る


Out put team

実験ノート


Time Responce Test


>Time Responce Liquid
>Time Responce Solid



ホーム メンバー紹介 プロジェクト紹介 作成したDNAパーツ ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B3"