Team:Mississippi State/Try

From 2008.igem.org

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<span style="background-color: lime"> Sam i got something.</span>
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<tr><td bgcolor="lightgreen"><SPAN style="color:fuchsia">'''iGEM?'''</SPAN><p><SPAN style="color:midnightblue">Hundreds of undergraduates all over the world spend their summer making Synthetic Biology a reality by participating in the annual International Genetically Engineered Machine competition.</SPAN></td></tr>
 
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<tr><td align=center>$1.40</td></tr>
 
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== Box types ==
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{| border="1" cellspacing="5" cellpadding="5" bgcolor="white" width="79%"
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!Type
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!Example
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|-
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! type=search
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|
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<inputbox>
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type=search
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</inputbox>
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|-
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| colspan="2" | Generates a search box with a default width of 50 characters. The button labels can be specified, see below; defaults [[MediaWiki:Tryexact]] and [[MediaWiki:Searchfulltext]]. The text field specifies the search criteria.
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|-
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! type=create
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|
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<inputbox>
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type=create
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</inputbox>
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|-
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| colspan="2" | Allows creating a new page. The button label can be specified, see below; default [[MediaWiki:Createarticle]]. The text field specifies which page is to be edited.
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|-
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! type=comment
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|
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<inputbox>
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type=comment
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</inputbox>
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|-
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| colspan="2" | Add one or more sections to an existing page, or create a page, with an edit summary that is automatically the same as the (first) new section header. The button label can be specified, see below; default [[MediaWiki:Postcomment]] also used in the sidebar of the "Standard" skin.  The text field specifies which page is to be edited.
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|}
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<div style="clear:both;"></div>
{| style="color:#000000;background-color:#ccff99;" cellpadding="1" cellspacing="0" border="0" bordercolor="#fff" width="75%"  align="right"
{| style="color:#000000;background-color:#ccff99;" cellpadding="1" cellspacing="0" border="0" bordercolor="#fff" width="75%"  align="right"
!align="left"|
!align="left"|
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=Mississippi State University Synthetic Biology=
=Mississippi State University Synthetic Biology=
You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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{| style="color:#1b2c8a;background-color:#ccff99;" cellpadding="1" cellspacing="0" border="0" bordercolor="#fff" width="25%"  align="left"
{| style="color:#1b2c8a;background-color:#ccff99;" cellpadding="1" cellspacing="0" border="0" bordercolor="#fff" width="25%"  align="left"
!align="left"|
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[[Image:Msusb logo.JPG|225 px|center]]
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[[Image:Msusb logo.JPG|230 px|center]]
*[[Team:Mississippi_State|Home]]
*[[Team:Mississippi_State|Home]]
*[[Team:Mississippi State/Team|The Team]]
*[[Team:Mississippi State/Team|The Team]]
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*[http://www.uni-bonn.de/~manfear/html2wiki-tables.php HTML to WIKI Converter]
*[http://www.uni-bonn.de/~manfear/html2wiki-tables.php HTML to WIKI Converter]
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{| style="color:#000000;background-color:"#ffff33";" cellpadding="1" cellspacing="0" border="0" bordercolor="#fff" width="75%"  align="right"
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!align="left"|
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This is the content of our page!!!
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{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 825px; color: #000000; background-color: #ffffff;"
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|-valign="top"
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<h3>Project Goals</h3>
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# Fuse GFPuv to GhR1 (the gene corresponding with our E3 of choice).<br>
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# Clone GhR1-GFPuv gene into plasmid.<br>
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# Sequence to check.<br>
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# Fuse RFP to ubiquitin.<br>
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# Clone RFP-ubiquitin gene into separate plasmid.<br>
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# Sequence to check.<br>
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# Express proteins in the cell.  They will interact with each other and the poly-ubiquitin chain will grow.<br>
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# Lyse the cells.<br>
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# Add components for binding.<br>
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# Run gel.<br>
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# Analyze gel under UV light.<br>
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* If the protein band is tagged with GFPuv, it will be visible.  The expected outcome of expression of both GFPuv and RFP is amber colored light.
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Alternatives to co-transforming 2 plasmids into the cell:<br>
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a)  Perform 2 transformations in 1 pET plasmid.<br>
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b)  Find another plasmid that has a location to clone RFP into.
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[http://www.abe.msstate.edu/wiki/msusbcwiki/index.php/Problems<br>'''Problems Encountered + Solutions''']
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* June 22 - Ubiquitin-J01095 Construction
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* July 6 - Low GhR1 Expression in Protein Gel
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* July 23 - Unsuccessful Amplification of Ubiquitin
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* July 25 - Lack of Protein Separation
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* July 31 - Incomplete Protein Expression
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* August 8 - Restriction Site Within GhR1 Gene
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* August 28 - Incorrect GhR1 Sequence
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Latest revision as of 13:56, 7 July 2008

Today's Featured Article
In the News 		 

Did you know?
On this day

  Picture of the Day
About Wikipedia 		  Navigation
More Information







Box types

Type Example
type=search

<inputbox> type=search </inputbox>

Generates a search box with a default width of 50 characters. The button labels can be specified, see below; defaults MediaWiki:Tryexact and MediaWiki:Searchfulltext. The text field specifies the search criteria.
type=create

<inputbox> type=create </inputbox>

Allows creating a new page. The button label can be specified, see below; default MediaWiki:Createarticle. The text field specifies which page is to be edited.
type=comment

<inputbox> type=comment </inputbox>

Add one or more sections to an existing page, or create a page, with an edit summary that is automatically the same as the (first) new section header. The button label can be specified, see below; default MediaWiki:Postcomment also used in the sidebar of the "Standard" skin. The text field specifies which page is to be edited.

Mississippi State University Synthetic Biology

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Msusb logo.JPG

This is the content of our page!!!

Retrieved from "http://2008.igem.org/Team:Mississippi_State/Try"