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Paris/July 13
From 2008.igem.org
(Difference between revisions)
| Line 19: | Line 19: | ||
[[Remark :]] | [[Remark :]] | ||
| - | * Biobricks 2008 : use of the maximum recoverable volume minipreps | + | * Biobricks 2008 : use of the maximum recoverable volume of the minipreps |
| - | * Biobricks 2007 : use of 3µl of minipreps | + | * Biobricks 2007 : use of 3µl of the minipreps |
| Line 31: | Line 31: | ||
|- | |- | ||
|MP1 (= J23100) | |MP1 (= J23100) | ||
| - | |ok | + | |align="center"|ok |
|- | |- | ||
|MP2 (= J23107) | |MP2 (= J23107) | ||
| - | |ok | + | |align="center"|ok |
|- | |- | ||
|MP3 (= B0015) | |MP3 (= B0015) | ||
| - | |ok | + | |align="center"|ok |
|- | |- | ||
|MP5 (= JB0030) | |MP5 (= JB0030) | ||
| - | |ok | + | ||align="center"|ok |
|- | |- | ||
|MP6 (= E0422) | |MP6 (= E0422) | ||
| - | |ok | + | |align="center"|ok |
|} | |} | ||
| Line 63: | Line 63: | ||
|align="center"|19N P1 | |align="center"|19N P1 | ||
|- | |- | ||
| - | |4F_SP 1013 | + | |align="center"|4F_SP 1013 |
|align="center"|C0079 | |align="center"|C0079 | ||
|align="center"|K | |align="center"|K | ||
| Line 73: | Line 73: | ||
|align="center"|6H P1 | |align="center"|6H P1 | ||
|- | |- | ||
| - | |6D_SP 1007 | + | |align="center"|6D_SP 1007 |
|align="center"|S03879 | |align="center"|S03879 | ||
|align="center"|A | |align="center"|A | ||
| Line 108: | Line 108: | ||
|align="center"|23E P1 | |align="center"|23E P1 | ||
|- | |- | ||
| - | |7G_SP 1013 | + | |align="center"|7G_SP 1013 |
|align="center"|E1010 | |align="center"|E1010 | ||
|align="center"|K | |align="center"|K | ||
|align="center"|15M P2 | |align="center"|15M P2 | ||
|- | |- | ||
| - | |7E_SP 1001 | + | |align="center"|7E_SP 1001 |
|align="center"|E0040 | |align="center"|E0040 | ||
|align="center"|A | |align="center"|A | ||
|align="center"|5H P1 | |align="center"|5H P1 | ||
|- | |- | ||
| - | |2F_SP 1016 | + | |align="center"|2F_SP 1016 |
|align="center"|E0030 | |align="center"|E0030 | ||
|align="center"|K | |align="center"|K | ||
|align="center"|7G P1 | |align="center"|7G P1 | ||
|} | |} | ||
Revision as of 18:37, 15 July 2008
delete
Preparation of Competent Cells
- Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
- Culture at 37°C until DO=0,57 (around 3h)
- Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
- Spin during 8', 2000rpm, 4°C
- Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
- Spin during 8', 2000rpm, 4°C
- Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
- 1h of delay unexpected (problem with DNA lyophilized)
- Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C durin 40")
- Incubation O/N at 37°C of the transformed bacteria
- Biobricks 2008 : use of the maximum recoverable volume of the minipreps
- Biobricks 2007 : use of 3µl of the minipreps
Biobricks 2007
| Strain | Transformation |
| MP1 (= J23100) | ok |
| MP2 (= J23107) | ok |
| MP3 (= B0015) | ok |
| MP5 (= JB0030) | align="center"|ok |
| MP6 (= E0422) | ok |
Biobricks 2008
| details | Strain | Antibio | Plate 2007 |
| 2E_SP 1000 | B0034 | A | 3O P1 |
| 10F_SP 1001 | R0079 | A | 19N P1 |
| 4F_SP 1013 | C0079 | K | 7K P2 |
| 6H_SP 1001 | C0179 | A | 6H P1 |
| 6D_SP 1007 | S03879 | A | / |
| 6B_SP 1001 | S03154 | A | 24K P1 |
| 10F_SP 1002 | J23101 | A | 21G P3 |
| 11F_SP 1002 | J23109 | A | 23G P3 |
| 4C_SP 1000 | R0040 | A | 7O P1 |
| 2G_SP 1000 | C0040 | A | 5C P1 |
| 1C_SP 1008 | PSB1A3 | A | 23E P1 |
| 7G_SP 1013 | E1010 | K | 15M P2 |
| 7E_SP 1001 | E0040 | A | 5H P1 |
| 2F_SP 1016 | E0030 | K | 7G P1 |
