Team:Hawaii/Protocols

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(List of Protocols)
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== List of Protocols ==
== List of Protocols ==
This is a list of tried and true protocols used by our team.
This is a list of tried and true protocols used by our team.
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===''In vitro''===
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* [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]]
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* [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]]
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* [[DNA restriction digest]]
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* [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]
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* [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
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===''E. coli''===
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* [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]]
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
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* [[Team:Hawaii/Protocols/Plasmid_prep|Alkaline lysis / plasmid isolation]]
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* [[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]
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=== ''Synechocystis'' sp. PCC6803===
* [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]]
* [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]]
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* Alkaline lysis / plasmid isolation
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=== Media===
* [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]]
* [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]]
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:* LB
 
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:* LB + amp<sub>100</sub>
 
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:* LB + kan<sub>20</sub> + amp <sub>50</sub>
 
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:* LB + sp<sub>100</sub>
 
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:* LB + sp<sub>100</sub> + sm<sub>50</sub>
 
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:* BG-11 + 5% LB
 
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:* BG-11
 
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:* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub>
 
* Liquid growth media
* Liquid growth media
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* [[Team:Hawaii/Protocols/Antibiotics|Antibiotics]]
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=== BioBrick ===
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* [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
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* [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
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* [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
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* [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}
__NOTOC__
__NOTOC__

Latest revision as of 23:52, 18 July 2008

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List of Protocols

This is a list of tried and true protocols used by our team.

In vitro

E. coli

Synechocystis sp. PCC6803

Media

BioBrick

  • [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
  • [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
  • [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
  • [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]