Team:Hawaii/Protocols

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Latest revision as of 23:52, 18 July 2008

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This is a list of tried and true protocols used by our team.

[http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
[http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
[http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 4: / Line 4: @@
 == List of Protocols ==
 This is a list of tried and true protocols used by our team.
+===''In vitro''===
+* [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]]
+* [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]]
+* [[DNA restriction digest]]
+* [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]
+* [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
 ===''E. coli''===
 * [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]]
 * [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
 * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
-* Alkaline lysis / plasmid isolation
+* [[Team:Hawaii/Protocols/Plasmid_prep|Alkaline lysis / plasmid isolation]]
+* [[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]
 === ''Synechocystis'' sp. PCC6803===
@@ Line 15: / Line 23: @@
 === Media===
 * [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]]
-:* LB
-:* LB + amp<sub>100</sub>
-:* LB + kan<sub>20</sub> + amp <sub>50</sub>
-:* LB + sp<sub>100</sub>
-:* LB + sp<sub>100</sub> + sm<sub>50</sub>
-:* BG-11 + 5% LB
-:* BG-11
-:* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub>
 * Liquid growth media
+* [[Team:Hawaii/Protocols/Antibiotics|Antibiotics]]
 === BioBrick ===