Team:Hawaii/Protocols

From 2008.igem.org

(Difference between revisions)
(Media)
(In vitro)
 
(5 intermediate revisions not shown)
Line 4: Line 4:
== List of Protocols ==
== List of Protocols ==
This is a list of tried and true protocols used by our team.
This is a list of tried and true protocols used by our team.
 +
===''In vitro''===
 +
* [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]]
 +
* [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]]
 +
* [[DNA restriction digest]]
 +
* [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]
 +
* [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
 +
===''E. coli''===
===''E. coli''===
* [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]]
* [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]]
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
-
* Alkaline lysis / plasmid isolation
+
* [[Team:Hawaii/Protocols/Plasmid_prep|Alkaline lysis / plasmid isolation]]
 +
* [[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]
=== ''Synechocystis'' sp. PCC6803===
=== ''Synechocystis'' sp. PCC6803===

Latest revision as of 23:52, 18 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)


List of Protocols

This is a list of tried and true protocols used by our team.

In vitro

E. coli

Synechocystis sp. PCC6803

Media

BioBrick

  • [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
  • [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
  • [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
  • [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]